Distribution and evolution of circular miniproteins in flowering plants

Gruber, Christian W., Elliott, Alysha G., Ireland, David C., Delprete, Piero G., Dessein, Steven, Goransson, Ulf, Trabi, Manuela, Wang, Conan K., Kinghorn, Andrew B., Robbrecht, Elmar and Craik, David J. (2008) Distribution and evolution of circular miniproteins in flowering plants. The Plant Cell, 20 9: 2471-2483. doi:10.1105/tpc.108.062331


Author Gruber, Christian W.
Elliott, Alysha G.
Ireland, David C.
Delprete, Piero G.
Dessein, Steven
Goransson, Ulf
Trabi, Manuela
Wang, Conan K.
Kinghorn, Andrew B.
Robbrecht, Elmar
Craik, David J.
Title Distribution and evolution of circular miniproteins in flowering plants
Journal name The Plant Cell   Check publisher's open access policy
ISSN 1040-4651
Publication date 2008-09-01
Year available 2008
Sub-type Article (original research)
DOI 10.1105/tpc.108.062331
Open Access Status DOI
Volume 20
Issue 9
Start page 2471
End page 2483
Total pages 13
Place of publication Rockville, MD, U.S.
Publisher American Society of Plant Physiologists
Language eng
Subject 0607 Plant Biology
Abstract Cyclotides are disulfide-rich miniproteins with the unique structural features of a circular backbone and knotted arrangement of three conserved disulfide bonds. Cyclotides have been found only in two plant families: in every analyzed species of the violet family ( Violaceae) and in few species of the coffee family ( Rubiaceae). In this study, we analyzed >200 Rubiaceae species and confirmed the presence of cyclotides in 22 species. Additionally, we analyzed >140 species in related plant families to Rubiaceae and Violaceae and report the occurrence of cyclotides in the Apocynaceae. We further report new cyclotide sequences that provide insights into the mechanistic basis of cyclotide evolution. On the basis of the phylogeny of cyclotide-bearing plants and the analysis of cyclotide precursor gene sequences, we hypothesize that cyclotide evolution occurred independently in various plant families after the divergence of Asterids and Rosids (similar to 125 million years ago). This is strongly supported by recent findings on the in planta biosynthesis of cyclotides, which involves the serendipitous recruitment of ubiquitous proteolytic enzymes for cyclization. We further predict that the number of cyclotides within the Rubiaceae may exceed tens of thousands, potentially making cyclotides one of the largest protein families in the plant kingdom.
Formatted abstract
Cyclotides are disulfide-rich miniproteins with the unique structural features of a circular backbone and knotted arrangement of three conserved disulfide bonds. Cyclotides have been found only in two plant families: in every analyzed species of the violet family (Violaceae) and in few species of the coffee family (Rubiaceae). In this study, we analyzed >200 Rubiaceae species and confirmed the presence of cyclotides in 22 species. Additionally, we analyzed >140 species in related plant families to Rubiaceae and Violaceae and report the occurrence of cyclotides in the Apocynaceae. We further report new cyclotide sequences that provide insights into the mechanistic basis of cyclotide evolution. On the basis of the phylogeny of cyclotide-bearing plants and the analysis of cyclotide precursor gene sequences, we hypothesize that cyclotide evolution occurred independently in various plant families after the divergence of Asterids and Rosids (~125 million years ago). This is strongly supported by recent findings on the in planta biosynthesis of cyclotides, which involves the serendipitous recruitment of ubiquitous proteolytic enzymes for cyclization. We further predict that the number of cyclotides within the Rubiaceae may exceed tens of thousands, potentially making cyclotides one of the largest protein families in the plant kingdom.
Keyword Cyclic cystine knot
Cyclotide Kalata B1
Coffee family Rubiaceae
Oldenlandia-Affinis
Macrocyclic peptides
Seed plants
Phylogenetic-relationships
Chassalia parvifolia
Protein sequences
Structural motif
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Thu, 16 Apr 2009, 01:16:17 EST by Jennifer Greder on behalf of Institute for Molecular Bioscience