Cdx gene deficiency compromises embryonic hematopoiesis in the mouse

Wang, Yuan, Yabuuchi, Akiko, McKinney-Freeman, Shannon, Ducharme, Danica M. K., Ray, Manas K., Chawengsaksophak, Kallayanee, Archer, Trevor K. and Daley, George Q. (2008) Cdx gene deficiency compromises embryonic hematopoiesis in the mouse. PNAS: Proceedings of the National Academy of Sciences of the United States of America, 105 22: 7756-7761. doi:10.1073/pnas.0708951105


Author Wang, Yuan
Yabuuchi, Akiko
McKinney-Freeman, Shannon
Ducharme, Danica M. K.
Ray, Manas K.
Chawengsaksophak, Kallayanee
Archer, Trevor K.
Daley, George Q.
Title Cdx gene deficiency compromises embryonic hematopoiesis in the mouse
Formatted title
Cdx gene deficience compromises embryonic hemtopoiesis in the mouse
Journal name PNAS: Proceedings of the National Academy of Sciences of the United States of America   Check publisher's open access policy
ISSN 1091-6490
Publication date 2008-06-03
Year available 2008
Sub-type Article (original research)
DOI 10.1073/pnas.0708951105
Open Access Status DOI
Volume 105
Issue 22
Start page 7756
End page 7761
Total pages 6
Editor Randy Schekman
Place of publication Washing, DC, USA
Publisher National Acadamy of Sciences
Language eng
Subject C1
270202 Genome Structure
970106 Expanding Knowledge in the Biological Sciences
Abstract Cdx genes (Cdx1, Cdx2, and Cdx4) encode a family of caudal-related transcription factors that mediate anterior-posterior patterning during embryogenesis through Hox gene regulation. Homologues in the zebrafish have been shown to play key roles in blood formation. To define the role of Cdx genes during embryonic hematopoiesis in a mammalian system, we examined the hematopoietic potential of Cdx-deficient mouse embryonic stem cells (ESCs) in vitro and in vivo. Individual Cdx-deficient ESCs exhibited impaired embryonic hematopoietic progenitor formation and altered Hox gene expression, most notably for Cdx2 deficiency. A more severe hematopoietic defect was observed with compound Cdx deficiency than loss of function of any single Cdx gene. Reduced hematopoietic progenitor formation of ESCs deficient in multiple Cdx genes could be rescued by ectopic expression of Cdx4, concomitant with partially restored Hox gene expression. These results reveal an essential and partially redundant role for multiple Cdx genes during embryonic hematopoiesis in the mouse.
Formatted abstract
Cdx genes (Cdx1, Cdx2, and Cdx4) encode a family of caudal-related transcription factors that mediate anterior–posterior patterning during embryogenesis through Hox gene regulation. Homologues in the zebrafish have been shown to play key roles in blood formation. To define the role of Cdx genes during embryonic hematopoiesis in a mammalian system, we examined the hematopoietic potential of Cdx-deficient mouse embryonic stem cells (ESCs) in vitro and in vivo. Individual Cdx-deficient ESCs exhibited impaired embryonic hematopoietic progenitor formation and altered Hox gene expression, most notably for Cdx2 deficiency. A more severe hematopoietic defect was observed with compound Cdx deficiency than loss of function of any single Cdx gene. Reduced hematopoietic progenitor formation of ESCs deficient in multiple Cdx genes could be rescued by ectopic expression of Cdx4, concomitant with partially restored Hox gene expression. These results reveal an essential and partially redundant role for multiple Cdx genes during embryonic hematopoiesis in the mouse.
Keyword Hox genes
embryonic stem cell
A-P patterning
development
embryoid body
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online before print May 29, 2008

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
Institute for Molecular Bioscience - Publications
 
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Created: Wed, 15 Apr 2009, 21:55:25 EST by Jennifer Greder on behalf of Institute for Molecular Bioscience