Bovine sertoli cells colonize and form tubules in murine hosts following transplantation and grafting procedures

Zhang, Zhen, Hill, Jonathan, Holland, Michael, Kurihara, Yasuyuki and Loveland, Kate L. (2008) Bovine sertoli cells colonize and form tubules in murine hosts following transplantation and grafting procedures. Journal of Andrology, 29 4: 418-430. doi:10.2164/jandrol.107.004465


Author Zhang, Zhen
Hill, Jonathan
Holland, Michael
Kurihara, Yasuyuki
Loveland, Kate L.
Title Bovine sertoli cells colonize and form tubules in murine hosts following transplantation and grafting procedures
Journal name Journal of Andrology   Check publisher's open access policy
ISSN 0196-3635
Publication date 2008-07-01
Sub-type Article (original research)
DOI 10.2164/jandrol.107.004465
Open Access Status
Volume 29
Issue 4
Start page 418
End page 430
Total pages 13
Editor M. Hardy
P. Schlegel
Place of publication Schaumburg, IL, U.S.A.
Publisher American Society of Andrology
Language eng
Subject C1
070206 Animal Reproduction
830302 Dairy Cattle
Abstract The contribution of somatic cells to nonrodent male germ cell transplantation success has not been well established due to lack of cell type-specific markers to distinguish donor cells from host cells. In the present study, we first screened antibodies and a lectin to identify markers suitable for unequivocal distinction between germ cells and Sertoli cells in bovine testes compared with mouse testes. Anti-vimentin and the Dolichos biflorus agglutinin (DBA) lectin detected only bovine Sertoli cells and spermatogonia, respectively; anti-NONO and anti-GCNA1 detected only mouse Sertoli and germ cells, respectively. The outcome of transplanting bovine testis cells into nude mouse testes was then studied using these markers. Our results clearly showed that immature bovine Sertoli cells survive and colonize mouse testes at 2.5 months after transplantation and that tubular structures composed of donor Sertoli cells formed adjacent to murine tubules within the host mouse testis. Bovine germ cell colonization and survival in mouse testes after transplantation were confirmed, but this was restricted to areas of bovine Sertoli cell colonization. In addition, ectopic grafts of intact bovine testis tissue and cell aggregates from hanging drop cultures were placed under the back skin and testis capsule of nude mice. Bovine Sertoli cells in ectopic grafts and aggregates were able to form tubular structures, and some bovine germ cells were observed around 2 months after implantation. This study therefore identifies a practical strategy to assess the outcome of testicular cell transplantation using different antibodies and a lectin to distinguish bovine cells from mouse cells. It identifies an approach that can readily be adapted to study other nonrodent species.
Q-Index Code C1
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Veterinary Science Publications
 
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Created: Tue, 14 Apr 2009, 21:32:34 EST by Narelle Poole on behalf of School of Veterinary Science