Phospholipase C/Protein kinase C pathway Mediates Angiotensin ll-Dependent Apoptosis in Neonatal Rat cardiac Fibroblasts Expressing AT1 Receptor

Vivar, Raul, Soto, C, Copaja, M, Mateluna, F, Aranguiz, P, Munoz, J P, Chiong, M, Garcia, L, Letelier, A, Thomas, W G, Lavandero, S and Diaz-Araya, G (2008) Phospholipase C/Protein kinase C pathway Mediates Angiotensin ll-Dependent Apoptosis in Neonatal Rat cardiac Fibroblasts Expressing AT1 Receptor. Journal of Cardiovascular Pharmacology, 52 2: 184-190. doi:10.1097/FJC.0b013e318181fadd


Author Vivar, Raul
Soto, C
Copaja, M
Mateluna, F
Aranguiz, P
Munoz, J P
Chiong, M
Garcia, L
Letelier, A
Thomas, W G
Lavandero, S
Diaz-Araya, G
Title Phospholipase C/Protein kinase C pathway Mediates Angiotensin ll-Dependent Apoptosis in Neonatal Rat cardiac Fibroblasts Expressing AT1 Receptor
Journal name Journal of Cardiovascular Pharmacology   Check publisher's open access policy
ISSN 0160-2446
Publication date 2008-08-01
Year available 2008
Sub-type Article (original research)
DOI 10.1097/FJC.0b013e318181fadd
Open Access Status DOI
Volume 52
Issue 2
Start page 184
End page 190
Total pages 6
Editor DeStefano, F R
Place of publication United States
Publisher Lippincott Williams & Wilkins
Language eng
Subject C1
060103 Cell Development, Proliferation and Death
920103 Cardiovascular System and Diseases
Abstract Cardiac fibroblasts are the major non-myocyte cell constituent in the myocardium, and they are involved in heart remodeling. Angiotensin II type I receptor (AT(1)R), and changes in its expression have been reported in cardiac fibroblasts after myocardial infarction. However, the AT(1)R-dependent signaling pathways involved in cardiac fibroblast death remain unknown. Using adenovirus, we ectopically expressed AT(1)R in cultured neonatal rat cardiac fibroblasts and investigated the role of the phospholipase (PLC)/protein kinase C (PKC) pathway on Ang II-dependent death. Ang II induced cardiac fibroblast death characterized by an early loss of mitochondrial membrane potnetial, increased Bax/Bc1-2 ratio, caspase-3 activation, and DNA fragmentation. All these effects were prevented by the AT(1)R antagonist losartan, PLC inhibitor U73122, and PKC inhibitor G06976. We conclude that Ang II stimulates the intrinsic apoptotic pathway in cultured cardiac fibroblasts by the AT(1)R/PLC/PKC signaling pathway.
Keyword Angiotensin ll
AT
Receptor
Fibroblast
Apoptosis
Cell death
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Biomedical Sciences Publications
 
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Created: Tue, 14 Apr 2009, 19:44:13 EST by Shirley Rey on behalf of School of Biomedical Sciences