Role of phenylalanine ammonia-lyase in heat pretreatment-induced chilling tolerance in banana fruit

Chen, Jian-ye, He, Li-hong, Jiang, Yue-ming, Wang, Yong and Joyce, Daryl C. (2008) Role of phenylalanine ammonia-lyase in heat pretreatment-induced chilling tolerance in banana fruit. Physiologia Plantarum, 132 3: 318-328. doi:10.1111/j.1399-3054.2007.01013.x


Author Chen, Jian-ye
He, Li-hong
Jiang, Yue-ming
Wang, Yong
Joyce, Daryl C.
Title Role of phenylalanine ammonia-lyase in heat pretreatment-induced chilling tolerance in banana fruit
Journal name Physiologia Plantarum   Check publisher's open access policy
ISSN 0031-9317
Publication date 2008-03-01
Year available 2008
Sub-type Article (original research)
DOI 10.1111/j.1399-3054.2007.01013.x
Open Access Status Not yet assessed
Volume 132
Issue 3
Start page 318
End page 328
Total pages 11
Editor Hurry, Vaughn
Place of publication Sweden
Publisher Scandinavian Society for Plant Physiology
Language eng
Subject C1
820214 Tropical Fruit
060705 Plant Physiology
Abstract Increasing evidence suggests that phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) is associated with low temperature stress in plant tissues. Banana fruit are highly susceptible to chilling injury. However, little is known about the role of PAL (i.e. gene expression, protein level and activity) in fruit chilling. In this work, the involvement of PAL induced by heat treatment (38 degrees C for 3 days) prior to storage (8 degrees C) in chilling tolerance was investigated. The PAL inhibitor 2-aminoindan-2-phosphonic acid (AIP) was also used to further study the role of PAL in the chilling tolerance. The results showed that mRNA transcripts (MaPAL1 and MaPAL2) and PAL protein levels increased during storage at chilling temperature. Heat treatment prior to storage alleviated chilling injury and enhanced PAL activity, protein amount and MaPAL1 and MaPAL2 transcript levels. The increases in parameters of PAL upon heat pretreatment were all inhibited by AIP treatment, which resulted in aggravation of chilling injury. Thus, these findings indicate that the induction of PAL by heat pretreatment was regulated at both the transcriptional and the translational levels and that PAL may play a role in heat pretreatment-induced chilling tolerance of banana fruit.
Keyword Plant Sciences
Plant Sciences
PLANT SCIENCES
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Agriculture and Food Sciences
 
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Created: Fri, 10 Apr 2009, 00:17:02 EST by Emma Cushworth on behalf of School of Land, Crop and Food Sciences