Effect of variation in the chain length and number in modulating the interaction of an immunogenic lipopeptide with biomembrane models

Sarpietro, Maria Grazia, Micieli, Dorotea, Pignatello, Rosario, Liang, Ming Tao, Toth, Istvan and Castelli, Francesco (2008) Effect of variation in the chain length and number in modulating the interaction of an immunogenic lipopeptide with biomembrane models. Thermochimica Acta, 471 1-2: 14-19. doi:10.1016/j.tca.2008.02.006


Author Sarpietro, Maria Grazia
Micieli, Dorotea
Pignatello, Rosario
Liang, Ming Tao
Toth, Istvan
Castelli, Francesco
Title Effect of variation in the chain length and number in modulating the interaction of an immunogenic lipopeptide with biomembrane models
Journal name Thermochimica Acta   Check publisher's open access policy
ISSN 0040-6031
Publication date 2008-05-30
Sub-type Article (original research)
DOI 10.1016/j.tca.2008.02.006
Open Access Status
Volume 471
Issue 1-2
Start page 14
End page 19
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Language eng
Subject C1
030499 Medicinal and Biomolecular Chemistry not elsewhere classified
920109 Infectious Diseases
Abstract A differential scanning calorimetry study was carried out to investigate the effect exerted by immunogenic synthetic lipopeptides obtained by the conjugation of LCMV33–41 peptide with lipoamino acids (Laas) bearing different alkyl chain lengths (C12 and C16) and number of chains (2 × C12) on the thermotropic behaviour of dimyristoylphosphatidylcholine (DMPC) liposomes. The aim of this work was to study the ability of these compounds to be carried by a liposomal system and released to a biomembrane model. The examined compounds caused variations of the thermotropic parameters that characterise the liposomal system (transition temperature, Tm and enthalpy variation, ΔH), and interacted with the biomembrane models in different way. The interaction was found to be modulated by the length and number of chains present in the examined compounds. In fact, the compounds with higher number of lipid chain showed a stronger interaction with the biomembrane models with respect to the pure peptide and the compounds with a single lipid chain. These results suggest that the lipoamino acid moiety could favour the peptide to be carried by the liposomal system and released to biomembrane.
Keyword Lipopeptides
Dimyristolphosphatidylcoline
Differential scanning calorimetry
Liposomes
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 15 February 2008.

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
 
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Created: Thu, 09 Apr 2009, 22:47:57 EST by Jennifer Falknau on behalf of School of Chemistry & Molecular Biosciences