Involvement of Reactive Oxygen Stress in Cadmium-induced Cellular Damage in Euglena gracilis

Watanabe, Masumi and Suzuki, Tetsuya (2002) Involvement of Reactive Oxygen Stress in Cadmium-induced Cellular Damage in Euglena gracilis. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 131 4: 491-500. doi:10.1016/S1532-0456(02)00036-4


Author Watanabe, Masumi
Suzuki, Tetsuya
Title Involvement of Reactive Oxygen Stress in Cadmium-induced Cellular Damage in Euglena gracilis
Formatted title
Involvement of Reactive Oxygen Stress in Cadmium-induced Cellular Damage in Euglena gracilis

Journal name Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology   Check publisher's open access policy
ISSN 1532-0456
Publication date 2002-04-01
Sub-type Article (original research)
DOI 10.1016/S1532-0456(02)00036-4
Open Access Status
Volume 131
Issue 4
Start page 491
End page 500
Total pages 10
Editor Thomas P. Mommsen
Patrick J. Walsh
Place of publication New York
Publisher Elsevier
Language eng
Subject 060199 Biochemistry and Cell Biology not elsewhere classified
Formatted abstract
Inorganic cadmium (Cd) causes cellular damage to eukaryotes and to tissues of higher organisms, including DNA strand breaks and intracellular membrane damage, as a result of reactive oxygen stress. We previously reported cadmium chloride (CdCl2)-induced abnormal cell morphologies in the unicellular eukaryote Euglena gracilis Z (a plant cell model) and its achlorophyllous mutant SMZ strain (an animal cell model). The present study was undertaken to examine whether exposure of both strains to CdCl2 would lead to similar cellular responses, especially with regard to reactive oxygen stress loading and cellular damage. The results indicate that CdCl2 exposure can induce morphological alteration, linked to reactive oxygen stress. Both E. gracilis Z and SMZ cells subjected to short-term, high-dose CdCl2 exposure showed long ‘comet lengths’ in the so-called ‘Comet’ assay, indicating DNA strand breaks. Similarly, short-term, high-dose CdCl2-exposed cells and CdCl2-induced morphologically altered cells showed intense fluorescence of dihydrofluorescein (HFLUOR) after incubation with dihydrofluorescein diacetate (HFLUOR-DA). Positive data on the generation and involvement of intracellular reactive oxygen species (ROS) were obtained from long-term, low-dose CdCl2-exposed E. gracilis Z and SMZ, by thiobarbituric acid (TBA)–malondialdehyde (MDA) complex analyses.

Keyword Abnormal morphology
Cadmium exposure
DNA strand breaks
Euglena gracilis
Model organism
Oxidative stress
ROS
TBA–MDA complex
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes Formerly known as Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 43 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 44 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 09 Apr 2009, 21:40:19 EST by Juliette Grosvenor on behalf of Library Corporate Services