Decontamination of Aflatoxin-Forming Fungus and Elimination of Aflatoxin Mutagenicity with Electrolyzed NaCl Anode Solution

Suzuki, Tetsuya, Noro, Takashi, Kawamura, Yukio, Fukunaga, Kenji, Watanabe, Masumi, Ohta, Mari, Sugiue, Hisashi, Sato, Yuri, Kohno, Masahiro and Hotta, Kunimoto (2002) Decontamination of Aflatoxin-Forming Fungus and Elimination of Aflatoxin Mutagenicity with Electrolyzed NaCl Anode Solution. Journal of Agricultural and Food Chemistry, 50 3: 633-641. doi:10.1021/jf0108361


Author Suzuki, Tetsuya
Noro, Takashi
Kawamura, Yukio
Fukunaga, Kenji
Watanabe, Masumi
Ohta, Mari
Sugiue, Hisashi
Sato, Yuri
Kohno, Masahiro
Hotta, Kunimoto
Title Decontamination of Aflatoxin-Forming Fungus and Elimination of Aflatoxin Mutagenicity with Electrolyzed NaCl Anode Solution
Journal name Journal of Agricultural and Food Chemistry   Check publisher's open access policy
ISSN 0021-8561
1520-5118
Publication date 2002-01-01
Year available 2001
Sub-type Article (original research)
DOI 10.1021/jf0108361
Open Access Status
Volume 50
Issue 3
Start page 633
End page 641
Total pages 9
Editor James N. Seiber
Place of publication Easton, PA
Publisher American Chemical Society
Language eng
Subject 090899 Food Sciences not elsewhere classified
Formatted abstract
Electrolysis of a 0.1% (17.1 mM) solution of NaCl using separate anode and cathode compartments gives rise to solutions containing active chemical species. The strongly acidic “anode solution” (EW(+)) has high levels of dissolved oxygen and available chlorine in a form of hypochlorous acid (HOCl) with a strong potential for sterilization, which we have investigated here. Exposing Aspergillus parasiticus at an initial density of 103spores in 10 μL to a 50-fold volume (500 μL) of EW(+) containing ca. 390 μmol HOCl for 15 min at room temperature resulted in a complete inhibition of fungal growth, whereas the cathode solution (EW(−)) had negligible inhibitory effects. Moreover, the mutagenicity of aflatoxin B1 (AFB1) for Salmonella typhimurium TA-98 and TA-100 strains was strongly reduced after AFB1 exposure to the EW(+) but not with the EW(−). In high-performance liquid chromatography analysis, the peak corresponding to AFB1 disappeared after treatment with the EW(+), indicating decomposition of the aflatoxin. In contrast, the routinely used disinfectant sodium hypochlorite, NaOCl, of the same available chlorine content as that of EW(+) but in a different chemical form, hypochlorite (OCl-) ion, did not decompose AFB1 at pH 11. However, NaOCl did decompose AFB1 at pH 3, which indicated that the principle chemical formula to participate in the decomposition of AFB1 is not the OCl- ion but HOCl. Furthermore, because the decomposition of AFB1 was suppressed by pretreating the EW(+) with the OH radical scavenger thiourea, the chemical species responsible for the AFB1-decomposing property of the EW(+) should be at least due to the OH radical originated from HOCl. The OH in EW(+) was proved by electron spin resonance analysis.
Keyword Aspergillus parasiticus
Electrolyzed NaCl solution
Inactivation of aflatoxin B1
Mutagenicity
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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