UBF levels determine the number of active ribosomal RNA genes in mammals

Sanij, Elaine, Poortinga, Gretchen, Sharkey, Kerith, Hung, Sandy, Holloway, Timothy P., Ouin, Jaclyn, Robb, Elysia, Wong, Lee H., Thomas, Walter G., Stefanovsky, Victor, Moss, Tom, Rothblum, Lawrence, Hannan, Katherine M., McArthur, Grant A., Pearson, Richard B. and Hannan, Ross D. (2008) UBF levels determine the number of active ribosomal RNA genes in mammals. The Journal of Cell Biology, 183 7: 1259-1274. doi:10.1083/jcb.200805146

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Author Sanij, Elaine
Poortinga, Gretchen
Sharkey, Kerith
Hung, Sandy
Holloway, Timothy P.
Ouin, Jaclyn
Robb, Elysia
Wong, Lee H.
Thomas, Walter G.
Stefanovsky, Victor
Moss, Tom
Rothblum, Lawrence
Hannan, Katherine M.
McArthur, Grant A.
Pearson, Richard B.
Hannan, Ross D.
Title UBF levels determine the number of active ribosomal RNA genes in mammals
Journal name The Journal of Cell Biology   Check publisher's open access policy
ISSN 0021-9525
1540-8140
Publication date 2008-12-22
Year available 2008
Sub-type Article (original research)
DOI 10.1083/jcb.200805146
Open Access Status File (Publisher version)
Volume 183
Issue 7
Start page 1259
End page 1274
Total pages 16
Editor I. Mellman
Place of publication New York, NY, United States
Publisher Rockefeller University Press
Language eng
Abstract In mammals, the mechanisms regulating the number of active copies of the ~200 ribosomal RNA (rRNA) genes transcribed by RNA polymerase I are unclear. We demonstrate that depletion of the transcription factor upstream binding factor (UBF) leads to the stable and reversible methylation-independent silencing of rRNA genes by promoting histone H1–induced assembly of transcriptionally inactive chromatin. Chromatin remodeling is abrogated by the mutation of an extracellular signal-regulated kinase site within the high mobility group box 1 domain of UBF1, which is required for its ability to bend and loop DNA in vitro. Surprisingly, rRNA gene silencing does not reduce net rRNA synthesis as transcription from remaining active genes is increased. We also show that the active rRNA gene pool is not static but decreases during differentiation, correlating with diminished UBF expression. Thus, UBF1 levels regulate active rRNA gene chromatin during growth and differentiation.
Keyword Polymerase-I transcription
AG-NOR proteins
RDNA transcription
Saccharomyces-cerevisiae
Cell Differentiation
Chromatin structures
ERK phosphorylation
DNA-transcription
Splice Variants
Binding
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 5R01HL077814
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Biomedical Sciences Publications
 
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Created: Thu, 09 Apr 2009, 18:47:50 EST by Shirley Rey on behalf of Faculty of Science