Campylobacter jejuni response to human mucin MUC2: modulation of colonization and pathogenicity determinants

Tu, Quoc V., McGuckin, Michael A. and Mendz, George L. (2008) Campylobacter jejuni response to human mucin MUC2: modulation of colonization and pathogenicity determinants. Journal of Medical Microbiology, 57 7: 795-802. doi:10.1099/jmm.0.47752-0


Author Tu, Quoc V.
McGuckin, Michael A.
Mendz, George L.
Title Campylobacter jejuni response to human mucin MUC2: modulation of colonization and pathogenicity determinants
Formatted title
Campylobacter jejuni response to human mucin MUC2: modulation of colonization and pathogenicity determinants
Journal name Journal of Medical Microbiology   Check publisher's open access policy
ISSN 0022-2615
Publication date 2008-07-01
Year available 2008
Sub-type Article (original research)
DOI 10.1099/jmm.0.47752-0
Open Access Status Not yet assessed
Volume 57
Issue 7
Start page 795
End page 802
Total pages 8
Place of publication Reading, England
Publisher Society for General Microbiology
Language eng
Subject C1
970111 Expanding Knowledge in the Medical and Health Sciences
110309 Infectious Diseases
Abstract Campylobacter jejuni is the main cause of bacterial acute gastroenteritis worldwide. In its colonization of the host intestinal tract, it encounters secreted mucins in the mucus layer and surface mucins in the epithelial cells. Mucins are complex glycoproteins that comprise the major component of mucus and give mucus its viscous consistency. MUC2 is the most abundant secreted mucin in the human intestine; it is a major chemoattractant for C. jejuni, and the bacterium binds to it. There are no studies on the transcriptional response of the bacterium to this mucin. Here, cell-culture techniques and quantitative RT-PCR were used to characterize in vitro the effects of MUC2 on C. jejuni growth and the changes in expression of 20 C. jejuni genes related to various functions. The genes encoding cytolethal distending toxin protein (cdtABC), vacuolating cytotoxin (vacB), C. jejuni lipoprotein (jlpA), Campylobacter invasion antigen (ciaB), the multidrug efflux system (cmeAB), putative mucin-degrading enzymes (cj1344c, cj0843c, cj0256 and cj1055c), flagellin A (flaA) and putative rod-shape-determining proteins (mreB and mreC) were upregulated, whereas those encoding Campylobacter adhesion fibronectin-binding protein (cadF) and sialic acid synthase (neuB1) were downregulated. These results showed that C. jejuni utilizes MUC2 as an environmental cue for the modulation of expression of genes with various functions including colonization and pathogenicity.
Keyword Campylobacter Jejuni
MUC2
Pathogenicity determinants
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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Created: Thu, 09 Apr 2009, 01:28:11 EST by Joanne PRESTON on behalf of Faculty Of Health Sciences