Listeria monocytogenes internalins bind to the human intestinal mucin MUC2

Linden, Sara K., Bierne, Hélène, Sabet, Christophe, Png, Chin Wen, Florin, Timothy H., McGuckin, Michael A. and Cossart, Pascale (2008) Listeria monocytogenes internalins bind to the human intestinal mucin MUC2. Archives of Microbiology, 190 1: 101-104. doi:10.1007/s00203-008-0358-6


Author Linden, Sara K.
Bierne, Hélène
Sabet, Christophe
Png, Chin Wen
Florin, Timothy H.
McGuckin, Michael A.
Cossart, Pascale
Title Listeria monocytogenes internalins bind to the human intestinal mucin MUC2
Formatted title
Listeria monocytogenes internalins bind to the human intestinal mucin MUC2
Journal name Archives of Microbiology   Check publisher's open access policy
ISSN 0302-8933
1432-072X
Publication date 2008-07-01
Year available 2008
Sub-type Article (original research)
DOI 10.1007/s00203-008-0358-6
Volume 190
Issue 1
Start page 101
End page 104
Total pages 4
Place of publication Berlin / Heidelberg, Germany
Publisher Springer
Collection year 2009
Language eng
Subject C1
970111 Expanding Knowledge in the Medical and Health Sciences
110801 Medical Bacteriology
Abstract Listeria monocytogenes cross the intestinal barrier causing systemic infections with high mortality rates. Intestinal infection triggers release of intestinal mucus. We show that three L. monocytogenes internalins, InlB, InlC and InlJ all bound to MUC2 (the major component of intestinal mucus), but not to the cell surface mucin MUC1. Binding was strongest to InlB>InlC>InlJ (P < 0.001). Listerial internalins are characterized by their internalin domain, composed by leucine rich repeats (LRR) followed by an immunogloblin-like region. We report here that the internalin domain of the InlJ protein also bound MUC2, suggesting that an internalin domain is sufficient to bind to MUC2.
Keyword Listeria
LRR
Mucin
Mucus
MUC2
Muc1
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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Created: Thu, 09 Apr 2009, 01:14:55 EST by Joanne PRESTON on behalf of School of Medicine