Muc1 limits Helicobacter felis binding to gastric epithelial cells but does not limit colonization and gastric pathology following infection

Every, Alison L., Chionh, Yok-Teng, Skene, Caroline D., McGuckin, Michael A. and Sutton, Philip (2008) Muc1 limits Helicobacter felis binding to gastric epithelial cells but does not limit colonization and gastric pathology following infection. Helicobacter, 13 6: 489-493. doi:10.1111/j.1523-5378.2008.00644.x


Author Every, Alison L.
Chionh, Yok-Teng
Skene, Caroline D.
McGuckin, Michael A.
Sutton, Philip
Title Muc1 limits Helicobacter felis binding to gastric epithelial cells but does not limit colonization and gastric pathology following infection
Journal name Helicobacter   Check publisher's open access policy
ISSN 1523-5378
1083-4389
Publication date 2008-12-01
Sub-type Article (original research)
DOI 10.1111/j.1523-5378.2008.00644.x
Volume 13
Issue 6
Start page 489
End page 493
Total pages 5
Place of publication Malden, United States
Publisher Wiley-Blackwell
Collection year 2009
Language eng
Subject C1
970111 Expanding Knowledge in the Medical and Health Sciences
110801 Medical Bacteriology
Formatted abstract
BACKGROUND: The mucin Muc1 is constitutively expressed by the gastric mucosa and is likely the first point of direct contact between the host stomach and the adherent pathogens. The expression of Muc1 has been shown to limit colonization of mice by Helicobacter pylori, known to adhere to the gastric epithelium, as well as associated pathology. However, the potential role of this mucin against nonadherent Helicobacter has not been previously studied. We therefore examined the importance of Muc1 on the pathogenesis of Helicobacter felis, believed not to adhere to the murine mucosa. METHODS AND RESULTS: Using primary cell cultures, we found that H. felis can bind gastric epithelial cells in vitro, and adherence to epithelial cells deficient in Muc1 was increased compared to controls that expressed the mucin. However, following infection of deficient mice, we found that Muc1 did not impact on H. felis colonization or pathogenesis in vivo, in contrast to previous observations with H. pylori. CONCLUSIONS: This demonstrates a variable effect of Muc1 on protection against closely related adherent and nonadherent Helicobacter species, and supports a key role for Muc1 in limiting attachment of adherent bacteria to the gastric mucosal surface.
Keyword Helicobacter felis
Muc1
Colonization
Pathogenesis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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Created: Wed, 08 Apr 2009, 23:26:46 EST by Joanne PRESTON on behalf of Faculty Of Health Sciences