IgG Antibodies Against Common Gut Bacteria Are More Diagnostic for Crohn's Disease Than IgG Against Mannan or Flagellin

Adams, Rachel J., Heazlewood, Sharise P., Gilshenan, Kristen S., O'Brien, Mark, McGuckin, Michael A. and Florin, Timothy H. J. (2008) IgG Antibodies Against Common Gut Bacteria Are More Diagnostic for Crohn's Disease Than IgG Against Mannan or Flagellin. American Journal of Gastroenterology, 103 2: 386-396. doi:10.1111/j.1572-0241.2007.01577.x


Author Adams, Rachel J.
Heazlewood, Sharise P.
Gilshenan, Kristen S.
O'Brien, Mark
McGuckin, Michael A.
Florin, Timothy H. J.
Title IgG Antibodies Against Common Gut Bacteria Are More Diagnostic for Crohn's Disease Than IgG Against Mannan or Flagellin
Journal name American Journal of Gastroenterology   Check publisher's open access policy
ISSN 0002-9270
1572-0241
Publication date 2008-02-01
Year available 2007
Sub-type Article (original research)
DOI 10.1111/j.1572-0241.2007.01577.x
Open Access Status Not yet assessed
Volume 103
Issue 2
Start page 386
End page 396
Total pages 11
Place of publication New York USA
Publisher Blackwell Publishing; Nature Publishing Group
Language eng
Subject C1
970111 Expanding Knowledge in the Medical and Health Sciences
110307 Gastroenterology and Hepatology
110311 Medical Genetics (excl. Cancer Genetics)
Abstract INTRODUCTION: Antibodies to baker's yeast (mannan) have been widely used to aid in diagnosis of Crohn's disease. Recently, there has been interest in antibodies against a flagellin from Clostridium coccoides subphylum. We hypothesized that reactivity with these antigens is a surrogate marker for a generalized increased IgG response against intestinal microbiota in Crohn's disease.
Formatted abstract
INTRODUCTION: Antibodies to baker's yeast (mannan) have been widely used to aid in diagnosis of Crohn's disease. Recently, there has been interest in antibodies against a flagellin from Clostridium coccoides subphylum. We hypothesized that reactivity with these antigens is a surrogate marker for a generalized increased IgG response against intestinal microbiota in Crohn's disease. METHODS: We compared the diagnostic utility of IgG antibodies against flagellin and mannan with two complex surface antigen preparations, one derived from B. vulgatus (Bv), the other from over 20 common mucosa-associated microbiota, a multibacterial membrane preparation (MBP). IgG antibodies were measured in sera from two age- and sex-matched populations: 120 Crohn's patients (CD) and 160 gastroenterology controls (CON) comprising 40 ulcerative colitis (UC) and 120 non-IBD patients. RESULTS: IgG was elevated against all antigen preparations in Crohn's but statistical analysis of receiver operator characteristic (ROC) plots showed that IgG against the complex antigen preparations MBP and Bv had better diagnostic accuracy to distinguish the two populations (CD and CON) than IgG against mannan (P < or = 0.01) or flagellin (P < or = 0.04). Concentrations of antibody reactive with distinct individual antigens correlated weakly. DISCUSSION: The findings support our hypothesis that measurement of IgG reactivity against individual antigens gives an indication of a generalized increased IgG response against individual intestinal microbiota in Crohn's, rather than measuring specific immune responses important for pathogenesis. The data are consistent with either a mucosal defect that facilitates increased exposure to microbial antigens or an altered immune response, both of which could occur due to known genetic and molecular defects in Crohn's disease.
Keyword Gastroenterology & Hepatology
Gastroenterology & Hepatology
GASTROENTEROLOGY & HEPATOLOGY
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID R24DK64400
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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Created: Wed, 08 Apr 2009, 22:54:56 EST by Joanne PRESTON on behalf of Faculty Of Health Sciences