Biosynthesis of the Canine Zona Pellucida requires the integrated participation of both oocytes and granulosa cells

Blackmore, Daniel G., Baillie, Lucan R., Holt, Janet E., Dierkx, Lynda, Aitken, R. John and McLaughlin, Eileen A. (2004) Biosynthesis of the Canine Zona Pellucida requires the integrated participation of both oocytes and granulosa cells. Biology of Reproduction, 71 2: 661-668. doi:10.1095/biolreprod.104.028779


Author Blackmore, Daniel G.
Baillie, Lucan R.
Holt, Janet E.
Dierkx, Lynda
Aitken, R. John
McLaughlin, Eileen A.
Title Biosynthesis of the Canine Zona Pellucida requires the integrated participation of both oocytes and granulosa cells
Journal name Biology of Reproduction   Check publisher's open access policy
ISSN 0006-3363
Publication date 2004-01-01
Year available 2004
Sub-type Article (original research)
DOI 10.1095/biolreprod.104.028779
Open Access Status Not yet assessed
Volume 71
Issue 2
Start page 661
End page 668
Total pages 8
Place of publication Madison, WI
Publisher Society for the Study of Reproduction
Language eng
Subject 060702 Plant Cell and Molecular Biology
060803 Animal Developmental and Reproductive Biology
Abstract In the dog, attempts to localize the expression of zona pellucida (ZP) proteins during folliculogenesis have failed to demonstrate conclusively whether any or all of the zona proteins are synthesized in the oocyte or the granulosa cells. Probing of paraformaldehyde-fixed prepubertal canine ovarian tissue sections with a panel of fluorescently conjugated lectins localized the expression of glycoproteins during folliculogenesis. We confirm that six lectins (PSA, s-WGA, ECL, GSL-II, LEL, and STL) consistently labeled the ZP and adjacent granulosa cells of the developing follicle and that canine ZP expresses β-gal(1,4)glcNAc, β-gal(1,3)galNac, α-mannose, and terminal sialic acid residues in a developmentally specific manner. Riboprobes for canine ZPA and ZPC genes were produced and used for in situ hybridization studies of mRNA expression in canine folliculogenesis. In addition, we isolated a partial cDNA transcript from total ovarian RNA for the canine ZPB gene having a high degree of sequence identity with the felid and porcine ZPB homologues. Subsequently, the ZPA gene transcripts were localized to the cytoplasm of oocytes in primordial, primary, and early secondary follicles. We then localized expression of ZPB and ZPC gene transcripts to the granulosa cells of growing follicles, but not in squamous granulosa cells of primordial follicles or oocytes. These observations indicate that in the juvenile canine ovary, the oocyte is responsible for synthesis of the ZPA protein and directing synthesis of the ZPB and ZPC proteins by the granulosa cells and that ZP gene transcription occurs in a sequential manner during folliculogenesis.
Keyword Follicle
Granulosa cells
Oocyte development
Ovary
Q-Index Code C1
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Queensland Brain Institute Publications
 
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Created: Wed, 08 Apr 2009, 21:50:09 EST by Ms Sarada Rao on behalf of Queensland Brain Institute