Oxidative stress-induced cellular damage caused by UV and methyl viologen in Euglena gracilis and its suppression with rutin

Palmer, Helen, Ohta, Mari, Watanabe, Masumi and Suzuki, Tetsuya (2002) Oxidative stress-induced cellular damage caused by UV and methyl viologen in Euglena gracilis and its suppression with rutin. Journal of Photochemistry and Photobiology B: Biology, 67 2: 116-129. doi:10.1016/S1011-1344(02)00271-3


Author Palmer, Helen
Ohta, Mari
Watanabe, Masumi
Suzuki, Tetsuya
Title Oxidative stress-induced cellular damage caused by UV and methyl viologen in Euglena gracilis and its suppression with rutin
Journal name Journal of Photochemistry and Photobiology B: Biology   Check publisher's open access policy
ISSN 1011-1344
Publication date 2002-06-01
Sub-type Article (original research)
DOI 10.1016/S1011-1344(02)00271-3
Open Access Status Not yet assessed
Volume 67
Issue 2
Start page 116
End page 129
Total pages 14
Place of publication Lausanne
Publisher Elsevier Sequoia
Language eng
Subject 060199 Biochemistry and Cell Biology not elsewhere classified
Abstract The effects of ultraviolet radiation (UV-A: 320–400 nm and UV-B: 280–320 nm) and methyl viologen (MV) single or combined exposure, on the cell growth, viability and morphology of two strains of the unicellular flagellate Euglena gracilis, using the Z strain as a plant model and the achlorophyllous mutant SMZ strain as an animal model were investigated. Cell growth was not affected by MV only, whereas UV-A or UV-B single and combined exposure with MV inhibited the cell growth or decreased the viability. The SMZ strain had a higher number of abnormal cells than the Z strain after the third dose of UV-B was delivered simultaneously with MV. The abnormal cell number decreased when E. gracilis SMZ cells were preincubated with 100 μM rutin prior to the UV-B and MV exposure. There were higher abnormal cell numbers with groups exposed to UV rather than MV single exposure. Combined exposure to UV-B and 200 μM MV induced the highest levels of TBARS in both strains, and with the supplementation of rutin these high levels were suppressed. These results suggest that UV-A or UV-B irradiation alone or combined with MV cause considerable oxidative damage in E. gracilis cells, and rutin supplementation may suppress their adverse effects.
Keyword Euglena
Methyl viologen
Oxidative stress
Rutin
TBARS
Ultraviolet ray irradiation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
National Research Centre for Environmental Toxicology Publications
 
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Created: Wed, 08 Apr 2009, 21:39:55 EST by Ms Sarada Rao on behalf of National Res Centre For Environmental Toxicology