Phenotypic Characterization of Two Ancylostoma caninum Isolates with Different Susceptibilities to the Anthelmintic Pyrantel

Kopp, Steven R., Coleman, Glen T., McCarthy, James S. and Kotze, Andrew C. (2008) Phenotypic Characterization of Two Ancylostoma caninum Isolates with Different Susceptibilities to the Anthelmintic Pyrantel. Antimicrobial Agents and Chemotherapy, 52 11: 3980-3986. doi:10.1128/AAC.00523-08

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Author Kopp, Steven R.
Coleman, Glen T.
McCarthy, James S.
Kotze, Andrew C.
Title Phenotypic Characterization of Two Ancylostoma caninum Isolates with Different Susceptibilities to the Anthelmintic Pyrantel
Formatted title
Phenotypic Characterization of Two Ancylostoma caninum Isolates with Different Susceptibilities to the Anthelmintic Pyrantel
Journal name Antimicrobial Agents and Chemotherapy   Check publisher's open access policy
ISSN 0066-4804
Publication date 2008-11-01
Year available 2008
Sub-type Article (original research)
DOI 10.1128/AAC.00523-08
Open Access Status File (Publisher version)
Volume 52
Issue 11
Start page 3980
End page 3986
Total pages 7
Editor G. M. Eliopoulos
Place of publication Washing, DC, United States
Publisher American Society for Microbiology
Language eng
Subject C1
070707 Veterinary Microbiology (excl. Virology)
970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
Abstract The anthelmintic pyrantel plays an important role in the control of gastrointestinal helminths of humans and domestic animals. Despite the demonstration of pyrantel resistance in several helminth species over the last 20 years, the resistance mechanism remains unclear. It has been hypothesized that resistance may arise as a consequence of changes to the relative proportions of subpopulations of nicotinic acetylcholine receptors (nAchRs). To test this hypothesis, we examined the responses of two isolates of the canine hookworm Ancylostoma caninum with low-level resistance (isolate NT) and high-level resistance (isolate PR) to pyrantel to nicotinic agonist drugs reported to be selective for three nAchR subtypes. We used larval motility and conformation assays and force transduction experiments with adult worms. Pyrantel and levamisole were less potent against larvae of isolate PR than larvae of isolate NT (up to an 18-fold increase in the 50% inhibitory concentration); on the other hand, bephenium was more potent against larvae of isolate PR than larvae of isolate NT (twofold) and nicotine had the same potency against larvae of both isolates. In adults, pyrantel, levamisole, and nicotine were less potent against isolate PR than isolate NT (two- to threefold), but the potency of bephenium against the two isolates was equivalent. Our data indicate a complex pattern of nAchRs in this species and suggest that the two isolates differ in their relative sensitivities to agonists targeting different nAchRs.
Keyword CAENORHABDITIS-ELEGANS
CHOLINERGIC DRUGS
HUMAN HOOKWORM
GENE FAMILY
RESISTANCE
LEVAMISOLE
ASSAY
DOGS
PARASITOLOGY
CONTRACTION
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Veterinary Science Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 5 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 08 Apr 2009, 21:29:13 EST by Narelle Poole on behalf of School of Veterinary Science