Early patterning of the chorion leads to the trilaminar trophoblast cell structure in the placental labyrinth

Simmons, David G., Natale, David R. C., Begay, Valerie, Hughes, Martha, Leutz, Achim and Cross, James C. (2008) Early patterning of the chorion leads to the trilaminar trophoblast cell structure in the placental labyrinth. Development, 135 12: 2083-2091. doi:10.1242/dev.020099

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Author Simmons, David G.
Natale, David R. C.
Begay, Valerie
Hughes, Martha
Leutz, Achim
Cross, James C.
Title Early patterning of the chorion leads to the trilaminar trophoblast cell structure in the placental labyrinth
Journal name Development   Check publisher's open access policy
ISSN 0950-1991
Publication date 2008-01-01
Year available 2008
Sub-type Article (original research)
DOI 10.1242/dev.020099
Open Access Status File (Publisher version)
Volume 135
Issue 12
Start page 2083
End page 2091
Total pages 9
Place of publication Cambridge, United Kingdom
Publisher The Company of Biologists
Language eng
Subject C1
111404 Reproduction
839899 Environmentally Sustainable Animal Production not elsewhere classified
Abstract The labyrinth of the rodent placenta contains villi that are the site of nutrient exchange between mother and fetus. They are covered by three trophoblast cell types that separate the maternal blood sinusoids from fetal capillaries - a single mononuclear cell that is a subtype of trophoblast giant cell (sinusoidal or S-TGC) with endocrine function and two multinucleated syncytiotrophoblast layers, each resulting from cell-cell fusion, that function in nutrient transport. The developmental origins of these cell types have not previously been elucidated. We report here the discovery of cell-layer-restricted genes in the mid-gestation labyrinth (E12.5-14.5) including Ctsq in S-TGCs (also Hand1-positive), Syna in syncytiotrophoblast layer I (SynT-1), and Gcm1, Cebpa and Synb in syncytiotrophoblast layer II (SynT-II). These genes were also expressed in distinct layers in the chorion as early as E8.5, prior to villous formation. Specifically, Hand1 was expressed in apical cells lining maternal blood spaces (Ctsq is not expressed until E12.5), Syna in a layer immediately below, and Gcm1, Cebpa and Synb in basal cells in contact with the allantois. Cebpa and Synb were co-expressed with Gcm1 and were reduced in Gcm1 mutants. By contrast, Hand1 and Syna expression was unaltered in Gcm1 mutants, suggesting that Gcm1-positive cells are not required for the induction of the other chorion layers. These data indicate thatthe three differentiated trophoblast cell types in the abyrinth arise from distinct and autonomous precursors in the chorion that are patterned before morphogenesis begins.
Keyword Chorion
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 37934
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Biomedical Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 112 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 120 times in Scopus Article | Citations
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Created: Wed, 08 Apr 2009, 19:30:47 EST by Shirley Rey on behalf of School of Biomedical Sciences