Essential Role of Interleukin-12 (IL-12) and IL-18 for Gamma Interferon Production Induced by Listeriolysin O in Mouse Spleen Cells

Nomura, Takamasa, Kawamura, Ikuo, Tsuchiya, Kohsuke, Kohda, Chikara, Baba, Hisashi, Ito, Yutaka, Kimoto, Terumi, Watanabe, Isao and Mitsuyama, Masao (2002) Essential Role of Interleukin-12 (IL-12) and IL-18 for Gamma Interferon Production Induced by Listeriolysin O in Mouse Spleen Cells. Infection and Immunity, 70 3: 1049-1055. doi:10.1128/IAI.70.3.1049-1055.2002

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Author Nomura, Takamasa
Kawamura, Ikuo
Tsuchiya, Kohsuke
Kohda, Chikara
Baba, Hisashi
Ito, Yutaka
Kimoto, Terumi
Watanabe, Isao
Mitsuyama, Masao
Title Essential Role of Interleukin-12 (IL-12) and IL-18 for Gamma Interferon Production Induced by Listeriolysin O in Mouse Spleen Cells
Journal name Infection and Immunity
ISSN 1098-5522
1070-6313
Publication date 2002-03-01
Sub-type Article (original research)
DOI 10.1128/IAI.70.3.1049-1055.2002
Open Access Status File (Publisher version)
Volume 70
Issue 3
Start page 1049
End page 1055
Total pages 7
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract The mechanism of gamma interferon (IFN-{gamma}) production induced by listeriolysin O (LLO), a cytolytic virulence factor of Listeria monocytogenes, was analyzed with special reference to the involvement of macrophage-derived cytokines in spleen cells of mice. LLO purified from the culture supernatant of L. monocytogenes was capable of inducing a high level of IFN-{gamma} when its cytolytic activity was blocked by cholesterol treatment. The IFN-{gamma}-inducing ability of LLO was not dependent on possibly contaminating lipopolysaccharide. Depletion of CD11b+ cells resulted in a profound decrease in IFN-{gamma} production in response to LLO stimulation. Negative selection also suggested the contribution of DX5+ cells in IFN-{gamma} production. Reverse transcription-PCR revealed that expression of interleukin-12 (IL-12) p35 and p40 was induced by LLO but that the IL-18 mRNA level in the CD11b+ fraction of spleen cells was unchanged. There was no change in the expression of the IFN-{gamma}-inducing cytokine genes in the CD11b- fraction. Neutralization of IL-12 and IL-18 in culture abolished the IFN-{gamma} production almost completely. Spleen cells from IL-12- or IL-18-deficient mice never produced IFN-{gamma} after stimulation with LLO. These results clearly indicated that LLO, a well-known virulence factor of L. monocytogenes, is capable of inducing IFN-{gamma} from NK cells through induction of IL-12 and IL-18 from macrophages. LLO appeared to play essential roles, not only as a bacterial virulence factor but also as a bacterial modulin in the immune response of the host.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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Created: Wed, 08 Apr 2009, 01:45:43 EST by Ms Sarada Rao on behalf of Anaesthesiology and Critical Care - RBWH