Molecular trafficking mechanisms of multipotent mesenchymal stem cells derived from human bone marrow and placenta

Brooke, Gary, Tong, Hui, Levesque, Jean-Pierre and Atkinson, Kerry (2008) Molecular trafficking mechanisms of multipotent mesenchymal stem cells derived from human bone marrow and placenta. Stem Cells and Development, 17 5: 929-940. doi:10.1089/scd.2007.0156

Author Brooke, Gary
Tong, Hui
Levesque, Jean-Pierre
Atkinson, Kerry
Title Molecular trafficking mechanisms of multipotent mesenchymal stem cells derived from human bone marrow and placenta
Journal name Stem Cells and Development   Check publisher's open access policy
ISSN 1547-3287
Publication date 2008-10-01
Year available 2008
Sub-type Article (original research)
DOI 10.1089/scd.2007.0156
Open Access Status
Volume 17
Issue 5
Start page 929
End page 940
Total pages 32
Place of publication New Rochelle, NY, USA
Publisher Mary Ann Liebert Inc
Language eng
Subject C1
970111 Expanding Knowledge in the Medical and Health Sciences
060103 Cell Development, Proliferation and Death
119999 Medical and Health Sciences not elsewhere classified
Abstract We compared potential trafficking mechanisms used by human (h) multipotent mesenchymal stem cells (MSC) derived from bone marrow (bm) or placenta (p). Both hbmMSC and hpMSC expressed a broad range of cell surface adhesion molecules including beta1-integrins (CD29) and CD44. Array data showed that both hbmMSC and hpMSC expressed mRNA for the cell adhesion molecules CD54 (ICAM-1), E-cadherin, CD166 (ALCAM), CD56 (NCAM), CD106 (VCAM-1), CD49a, b, c, e and f (integrins alpha1, 2, 3, 4 and 6), integrin alpha11, CD51 (integrin alphaV), and CD29 (integrins beta1). Functional binding of hpMSC, but not hbmMSC to VCAM-1 was demonstrated using recombinant chimeric constructs. Neither bone marrow nor placental MSC expressed ligands to endothelial selectins such as PSGL-1 or sialyl Lewis X (sLe(x)) carbohydrates and neither were able to bind functionally to chimeric constructs of the endothelial selectins CD62E (E-selectin) and CD62P (P-selectin). Furthermore, MSC expressed a restricted range of transferases necessary for expression of sLe(x), with no detectable expression of fucosyl transferases IV or VII. Placental MSC, but not hbmMSC, expressed mRNA for the chemokine receptors CCR1 and CCR3, and both hbmMSC and hpMSC expressed mRNA for CCR7, CCR8, CCR10, CCR11, CXCR4 and CXCR6. Intracellular chemokine receptor protein expression of CCR1, CCR3, CXCR3, CXCR4 and CXCR6 was detected in both hbmMSC and hpMSC. Cell surface expression of chemokine receptors was much more restricted with only CXCR6 displaying a strong signal on hbmMSC and hpMSC. Although cell surface expression of CXCR4 was not detected, MSC migrated in response to its ligand, CXCL12 (SDF-1). Thus, hbmMSC and hpMSC have an almost identical profile for cell surface adhesion and chemokine receptor molecules at the mRNA and protein levels. However, at the functional level, hpMSC likely utilise VLA-4-mediated binding in a superior manner to hbmMSC and thus may have superior engraftment properties to hbmMSC in vivo.
Keyword Cell & Tissue Engineering
Medicine, Research & Experimental
Cell Biology
Research & Experimental Medicine
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 123 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 141 times in Scopus Article | Citations
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Created: Tue, 07 Apr 2009, 23:18:02 EST by Joanne PRESTON on behalf of School of Medicine