The Use of Experimental Murine Models to Assess Novel Agents of Hematopoietic Stem and Progenitor Cell Mobilization

Herbert, K.E., Levesque, J-P., Haylock, D.N. and Prince, M. (2008) The Use of Experimental Murine Models to Assess Novel Agents of Hematopoietic Stem and Progenitor Cell Mobilization. Biology of Blood and Marrow Transplantation, 14 6: 603-621. doi:10.1016/j.bbmt.2008.02.003


Author Herbert, K.E.
Levesque, J-P.
Haylock, D.N.
Prince, M.
Title The Use of Experimental Murine Models to Assess Novel Agents of Hematopoietic Stem and Progenitor Cell Mobilization
Journal name Biology of Blood and Marrow Transplantation   Check publisher's open access policy
ISSN 1083-8791
Publication date 2008-01-01
Year available 2008
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1016/j.bbmt.2008.02.003
Open Access Status DOI
Volume 14
Issue 6
Start page 603
End page 621
Total pages 19
Place of publication New York , U.S.A.
Publisher Elsevier Science
Language eng
Subject C1
970111 Expanding Knowledge in the Medical and Health Sciences
970106 Expanding Knowledge in the Biological Sciences
060103 Cell Development, Proliferation and Death
119999 Medical and Health Sciences not elsewhere classified
Abstract The recent explosion in the understanding of the cellular and molecular mechanisms underlying hematopoietic stem and progenitor cell (HSPC) mobilization has facilitated development of novel therapeutic agents, targeted at improving mobilization kinetics as well as HSPC yield. With the development of new agents comes the challenge of choosing efficient and relevant preclinical studies for the testing of the HSPC mobilization efficacy of these agents. This article reviews the use of the mouse as a convenient small animal model of HSPC mobilization and transplantation, and outlines the range of murine assays that can be applied to assess novel HSPC mobilizing agents. Techniques to demonstrate murine HSPC mobilization are discussed, as well as the role of murine assays to confirm human HSPC mobilization, and techniques to investigate the biologic phenotype of HSPC mobilized by these novel agents. Technical aspects regarding mobilization regimens and control arms, and choice of experimental animals are also discussed.
Keyword Hematopoietic Stem Cells
Stem Cell Mobilization
transplantation
murine
Hematopoiesis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: 2009 Higher Education Research Data Collection
School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 4 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 5 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 07 Apr 2009, 22:33:07 EST by Joanne PRESTON on behalf of School of Medicine