Increased biomass yield of Lactococcus lactis during energetically limited growth and respiratory conditions

Koebmann, Brian, Blank, Lars Mathias, Solem, Christian, Petranovic, Dina, Nielsen, Lars K. and Jensen, Peter Ruhdal (2008) Increased biomass yield of Lactococcus lactis during energetically limited growth and respiratory conditions. Biotechnology & Applied Biochemistry, 50 Pt. 1: 25-33. doi:10.1042/BA20070132

Author Koebmann, Brian
Blank, Lars Mathias
Solem, Christian
Petranovic, Dina
Nielsen, Lars K.
Jensen, Peter Ruhdal
Title Increased biomass yield of Lactococcus lactis during energetically limited growth and respiratory conditions
Journal name Biotechnology & Applied Biochemistry   Check publisher's open access policy
ISSN 0885-4513
Publication date 2008-05-01
Year available 2007
Sub-type Article (original research)
DOI 10.1042/BA20070132
Open Access Status
Volume 50
Issue Pt. 1
Start page 25
End page 33
Total pages 9
Editor Parviz Shamlou
Place of publication London, U.K.
Publisher Portland Press
Language eng
Subject C1
1003 Industrial Biotechnology
970110 Expanding Knowledge in Technology
Abstract Lactococcus lactis is known to be capable of respiration under aerobic conditions in the presence of haemin. In the present study the effect of respiration on ATP production during growth on different sugars was examined. With glucose as the sole carbon source, respiratory conditions in L. lactis MG1363 resulted in only a minor increase, 21%, in biomass yield. Since ATP production through substrate-level phosphorylation was essentially identical with and without respiration, the increased biomass yield was a result of energy-saving under respiratory conditions estimated to be 0.4 mol of ATP/mol of glucose. With maltose as the energy source, the increase in biomass yield amounted to 51% compared with an aerobic culture that lacked haemin. This higher ATP yield was obtained by redirecting pyruvate metabolism from lactate to acetate production, and from savings through respiration. However, even after subtracting these contributions, approx. 0.3 mol of ATP/mol of glucose remained unaccounted for. A similar response to respiratory conditions (0.2 mol of ATP/mol of glucose) was observed in a mutant that had a decreased glucose uptake rate during growth on glucose caused by disruption of the PTSmannose (glucose/mannose-specific phosphotransferase system). Amino acid catabolism could be excluded as the source of the additional ATP. Since mutants without a functional H+-ATPase produced less ATP under sugar starvation and respiratory conditions, the additional ATP yield appears to come partly from energy saved on proton pumping through the H+-ATPase due to respiration and partly from a reversed function of the H+-ATPase towards oxidative phosphorylation. These results may contribute to the design and implementation of carbon-efficient high-cell-density cultures of this industrially important species of bacterium.
Keyword cytochrome
energy metabolism
Lactococcus lactis
redox cofactor metabolism
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published as Immediate Publication 10 September 2007

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 17 times in Thomson Reuters Web of Science Article | Citations
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