A single method for cryofixation and correlative light, electron microscopy and tomography of zebrafish embryos

Nixon, S. J., Webb, R. I., Floetenmeyer, M., Schieber, N., Lo, H. P. and Parton, R.G. (2009) A single method for cryofixation and correlative light, electron microscopy and tomography of zebrafish embryos. Traffic, 10 2: 131-136. doi:10.1111/j.1600-0854.2008.00859.x


Author Nixon, S. J.
Webb, R. I.
Floetenmeyer, M.
Schieber, N.
Lo, H. P.
Parton, R.G.
Title A single method for cryofixation and correlative light, electron microscopy and tomography of zebrafish embryos
Journal name Traffic   Check publisher's open access policy
ISSN 1398-9219
1600-0854
Publication date 2009-02-01
Year available 2008
Sub-type Article (original research)
DOI 10.1111/j.1600-0854.2008.00859.x
Volume 10
Issue 2
Start page 131
End page 136
Total pages 5
Place of publication Koebenhavn, Denmark
Publisher Blackwell Munksgaard
Collection year 2009
Language eng
Subject C1
060110 Receptors and Membrane Biology
970106 Expanding Knowledge in the Biological Sciences
0699 Other Biological Sciences
Abstract The zebrafish is a powerful vertebrate system for cell and development studies. In this study, we have optimized methods for fast freezing and processing of zebrafish embryos for electron microscopy (EM). WE show that in the absence of primary chemical fixation, excellent ultrastructure, preservation of green fluorescent protein (GP) fluorescence, immunogold labeling and electron tomography can be obtained using a single technique involving high-pressure freezing and embedding in Lowicryl resins at low temperature.
Keyword CLEM
GFP
Tomographic particle image velocimetry
Zebrafish
Cryofixation
Q-Index Code C1
Q-Index Status Confirmed Code
Additional Notes Online 20 November 2008

 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 74 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 79 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 07 Apr 2009, 20:56:42 EST by Cody Mudgway on behalf of Faculty of Science