Development of chemically defined medium for Mannheimia succiniciproducens based on its genome sequence

Song, Hyohak, Kim, Tae Yong, Choi, Bo-Kyeong, Choi, Seong Jun, Nielsen, Lars K., Chang, Ho Nam and Lee, Sang Yup (2008) Development of chemically defined medium for Mannheimia succiniciproducens based on its genome sequence. Applied Microbiology and Biotechnology., 79 2: 263-272. doi:10.1007/s00253-008-1425-2

Author Song, Hyohak
Kim, Tae Yong
Choi, Bo-Kyeong
Choi, Seong Jun
Nielsen, Lars K.
Chang, Ho Nam
Lee, Sang Yup
Title Development of chemically defined medium for Mannheimia succiniciproducens based on its genome sequence
Journal name Applied Microbiology and Biotechnology.   Check publisher's open access policy
ISSN 0175-7598
Publication date 2008-05-01
Year available 2008
Sub-type Article (original research)
DOI 10.1007/s00253-008-1425-2
Open Access Status Not yet assessed
Volume 79
Issue 2
Start page 263
End page 272
Total pages 10
Editor Alexander Steinbuechel
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Subject C1
100303 Fermentation
060114 Systems Biology
970106 Expanding Knowledge in the Biological Sciences
Abstract This study presents a novel methodology for the development of a chemically defined medium (CDM) using genome-scale metabolic network and flux balance analysis. The genome-based in silico analysis identified two amino acids and four vitamins as non-substitutable essential compounds to be supplemented to a minimal medium for the sustainable growth of Mannheimia succiniciproducens, while no substitutable essential compounds were identified. The in silico predictions were verified by cultivating the cells on a CDM containing the six non-substitutable essential compounds, and it was further demonstrated by observing no cell growth on the CDM lacking any one of the non-substitutable essentials. An optimal CDM for the enhancement of cell growth and succinic acid production, as a target product, was formulated with a single-addition technique. The fermentation on the optimal CDM increased the succinic acid productivity by 36%, the final succinic acid concentration by 17%, and the succinic acid yield on glucose by 15% compared to the cultivation using a complex medium. The optimal CDM also lowered the sum of the amounts of by-products (acetic, formic, and lactic acids) by 30%. The strategy reported in this paper should be generally applicable to the development of CDMs for other organisms, whose genome sequences are available.
Keyword Chemically defined medium
Mannheimia succiniciproducens
Metabolic network
Flux balance analysis
Succinic acid
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online: 1 April 2008

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 42 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 07 Apr 2009, 02:56:16 EST by Mrs Jennifer Brown on behalf of Aust Institute for Bioengineering & Nanotechnology