Directing phenotype of vascular smooth muscle cells using electrically stimulated conducting polymer

Rowlands, Andrew S. and Cooper-White, Justin J. (2008) Directing phenotype of vascular smooth muscle cells using electrically stimulated conducting polymer. Biomaterials, 29 34: 4510-4520. doi:10.1016/j.biomaterials.2008.07.052


Author Rowlands, Andrew S.
Cooper-White, Justin J.
Title Directing phenotype of vascular smooth muscle cells using electrically stimulated conducting polymer
Journal name Biomaterials   Check publisher's open access policy
ISSN 0142-9612
Publication date 2008-12-01
Year available 2008
Sub-type Article (original research)
DOI 10.1016/j.biomaterials.2008.07.052
Open Access Status Not yet assessed
Volume 29
Issue 34
Start page 4510
End page 4520
Total pages 11
Editor D.F. Williams
Place of publication Amsterdam, Netherlands
Publisher Elsevier Science
Language eng
Subject C1
060103 Cell Development, Proliferation and Death
970106 Expanding Knowledge in the Biological Sciences
Abstract Vascular smooth muscle cells (VSMCs) isolated from rabbit aorta and immortalised A7r5 cells were cultured on conducting polypyrrole (PPy) substrates and were subjected to a 50 μA sinusoidal electrical stimulation at 0.05, 5 and 500 Hz. These substrates were doped with hyaluronic acid and coated with collagen IV followed by Matrigel® in order to mimic the basement membrane and encourage cell attachment. Increased proliferation and expression of smooth muscle phenotype markers (smooth muscle α-actin and smooth muscle myosin heavy chain) were observed in cultures stimulated at 5 and 500 Hz. This increased proliferation and expression of contractile proteins were found to be significantly decreased when L-type voltage-gated calcium channels (VGCC) were blocked with the drug nifedipine. To the best of our knowledge, this is the first work that demonstrates that VSMCs cultured on a conducting polymer substrate and subject to electrical stimulation not only exhibit enhanced proliferation but can be simultaneously encouraged to increase contractile protein expression. This behaviour is somewhat contrary to the classical definition of smooth muscle contractile and synthetic phenotypes that, in general, requires a modulation in phenotype as a prerequisite for smooth muscle proliferation. This interesting result highlights both the inherent plasticity of vascular smooth muscle cells and the potential of electrical stimulation via conducting polymer substrates to manipulate their behaviour.
Keyword Smooth muscle cell
Electrical stimulation
Electroactive polymer
Cell activation
Cell proliferation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Tue, 07 Apr 2009, 00:28:54 EST by Mrs Jennifer Brown on behalf of Aust Institute for Bioengineering & Nanotechnology