High-resolution melt analysis for the detection of a mutation associated with permethrin resistance in a population of scabies mites

Pasay, C., Arlian, L., Morgan, M., Vyszenski-Moher, D., Rose, A., Holt, D., Walton, S. and McCarthy, J. S. (2008) High-resolution melt analysis for the detection of a mutation associated with permethrin resistance in a population of scabies mites. Medical and Veterinary Entomology, 22 1: 82-88. doi:10.1111/j.1365-2915.2008.00716.x


Author Pasay, C.
Arlian, L.
Morgan, M.
Vyszenski-Moher, D.
Rose, A.
Holt, D.
Walton, S.
McCarthy, J. S.
Title High-resolution melt analysis for the detection of a mutation associated with permethrin resistance in a population of scabies mites
Journal name Medical and Veterinary Entomology   Check publisher's open access policy
ISSN 1365-2915
0269-283X
Publication date 2008-03-01
Year available 2008
Sub-type Article (original research)
DOI 10.1111/j.1365-2915.2008.00716.x
Open Access Status Not yet assessed
Volume 22
Issue 1
Start page 82
End page 88
Total pages 7
Editor G. Gibson
R. Wall
Place of publication United Kingdom
Publisher Wiley- Blackwell Publishing Ltd
Language eng
Subject C1
730101 Infectious diseases
110309 Infectious Diseases
Abstract Permethrin as a topical acaricide cream is widely used to treat scabies. The neuronal voltage-sensitive sodium channel (Vssc), necessary for the generation of action potentials in excitable cells, is the target of pyrethroid acaricides such as permethrin. Pyrethroid resistance has been linked to specific mutations in the Vssc gene. Following the partial sequencing of the Vssc gene in the scabies mite Sarcoptes scabiei (L.) (Astigmata: Sarcoptidae), we compared Vssc gene sequences from permethrin-sensitive and -tolerant S. scabiei var. canis Gerlach mites, and identified a G to A single nucleotide polymorphism (SNP) in permethrin-tolerant mites resulting in an amino acid change from glycine to aspartic acid in domain III S6. The mutation is in a region of the gene where mutations have been identified in a range of pyrethroid-resistant arthropods. Results of in vitro permethrin exposure assays showed that survival rates for mites bearing the mutation were similar to those previously reported for mites from human subjects where clinical tolerance to permethrin had been observed. A real-time polymerase chain reaction−high-resolution melt (PCR-HRM) assay was developed to detect this SNP. This assay provides a useful methodology for screening for this and other mutations associated with permethrin resistance in scabies mite populations and thus facilitates surveillance for acaricide resistance.
Keyword High-resolution melt analysis
knockdown resistance
Permethrin
real-time PCR
scabies mite
Voltage-gated Sodium Channel
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Medicine Publications
 
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Created: Mon, 06 Apr 2009, 22:15:25 EST by Amy Wong on behalf of Medicine - Royal Brisbane and Women's Hospital