Practical blood dendritic cell vaccination for immunotherapy of multiple myeloma

Vari F, Munster D.J., Hsu J.L., Rossetti T.R., Stephen Mahler, Gray, Peter P., Turtle C.J., Prue R.L. and Hart D.N. (2008) Practical blood dendritic cell vaccination for immunotherapy of multiple myeloma. British Journal Of Haematology, 143 3: 374-377. doi:10.1111/j.1365-2141.2008.07346.x


Author Vari F
Munster D.J.
Hsu J.L.
Rossetti T.R.
Stephen Mahler
Gray, Peter P.
Turtle C.J.
Prue R.L.
Hart D.N.
Title Practical blood dendritic cell vaccination for immunotherapy of multiple myeloma
Journal name British Journal Of Haematology   Check publisher's open access policy
ISSN 0007-1048
Publication date 2008-01-01
Year available 2008
Sub-type Article (original research)
DOI 10.1111/j.1365-2141.2008.07346.x
Open Access Status Not yet assessed
Volume 143
Issue 3
Start page 374
End page 377
Total pages 4
Editor F Cotter, I Hann, J Reilly
Place of publication Oxford, U.K.
Publisher Blackwell Publishing Ltd
Language eng
Subject C1
111204 Cancer Therapy (excl. Chemotherapy and Radiation Therapy)
860803 Human Pharmaceutical Treatments (e.g. Antibiotics)
Abstract Therapeutic vaccination combined with new drugs may cure multiple myeloma (MM). We have developed a bio-process to purify CMRF-56 monoclonal antibody (mAb) and a standard operating procedure to immunoselect blood dendritic cells (BDC). Leucopheresed mononuclear cells were cultured overnight, labelled with CMRF-56 mAb and BDC prepared using a clinical scale immunoselection system. The mean BDC yield from healthy donors was 48% (n = 6, purity 28%). Preparations from MM patients (n = 6, yield 47%, purity 35%) primed cytotoxic T lymphocytes (CTL) to clinically relevant MM antigens. This procedure can be performed readily by clinical cell manufacturing units to facilitate BDC vaccination studies.
Keyword Hematology
Hematology
HEMATOLOGY
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Mon, 06 Apr 2009, 22:07:38 EST by Mrs Jennifer Brown on behalf of Aust Institute for Bioengineering & Nanotechnology