Subcellular compartment targeting of layered double hydroxide nanoparticles

Xu, Zhi Ping, Niebert, Marcus, Porazik, Katharina, Walker, Tara L., Cooper, Helen M., Middelberg, Anton P.J., Gray, Peter P., Bartlett, Perry F. and Lu, Gao Qing (Max) (2008) Subcellular compartment targeting of layered double hydroxide nanoparticles. Journal of Controlled Release, 130 1: 86-94. doi:10.1016/j.jconrel.2008.05.021

Author Xu, Zhi Ping
Niebert, Marcus
Porazik, Katharina
Walker, Tara L.
Cooper, Helen M.
Middelberg, Anton P.J.
Gray, Peter P.
Bartlett, Perry F.
Lu, Gao Qing (Max)
Title Subcellular compartment targeting of layered double hydroxide nanoparticles
Journal name Journal of Controlled Release   Check publisher's open access policy
ISSN 0168-3659
Publication date 2008-08-25
Year available 2008
Sub-type Article (original research)
DOI 10.1016/j.jconrel.2008.05.021
Open Access Status Not yet assessed
Volume 130
Issue 1
Start page 86
End page 94
Total pages 9
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Language eng
Subject C1
100708 Nanomaterials
860899 Human Pharmaceutical Products not elsewhere classified
Abstract Current investigations show that layered double hydroxide (LDH) nanoparticles have high potential as effective non-viral agents for cellular drug delivery due to their low cytotoxicity. good biocompatibility, high drug loading, control of particle size and shape, targeted delivery and drug release control. Two types of Mg2Al-LDH nanoparticles with fluorescein isothiocyanate (FITC) were controllably prepared. One is morphologically featured as typical hexagonal sheets (50-150 nm laterally wide and 10-20 nm thick), while the other as typical rods (30-60 nm wide and 100-200 nm long). These LDHFTIC nanoparticles are observed to immediately transfect into different mammalian cell lines. We found that internalized LDHFITC nanorods are quickly translocated into the nucleus while internalized LDHFITC nanosheets are retained in the cytoplasm. Inhibition experiments show that the cellular uptake is a clathrin-mediated time- and concentration-dependent endocytosis. Endosomal escape of LDHFITC nanoparticles is suggested to occur through the deacidification of LDH nanoparticles. Since quick nuclear targeting of LDHFITC nanorods requires an active process, and although the exact mechanism is yet to be fully understood, it probably involves an active transport via microtubule-mediated trafficking processes. Targeted addressing of two major subcellular compartments by simply controlling the particle morphology/size could find a number of applications in cellular biomedicine.
Keyword Layered Double Hydroxides
Cellular Uptake
Clathrin-mediated Endocytosis
Subcellular Compartment Targeting
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID DP0559594
Institutional Status UQ

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Created: Mon, 06 Apr 2009, 20:32:22 EST by Amanda Lee on behalf of Queensland Brain Institute