Maximum rate of oxygen consumption related to succinate dehydrogenase activity in skeletal muscle fibres of chronic heart failure patients and controls

Bekedam, Martijn A., van Beek-Harmsen, Brechje J., Boonstra, Anco, van Mechelen, Willem, Visser, Frans C. and van der Laarse, Willem J. (2003) Maximum rate of oxygen consumption related to succinate dehydrogenase activity in skeletal muscle fibres of chronic heart failure patients and controls. Clinical Physiology and Functional Imaging, 23 6: 337-343. doi:10.1046/j.1475-0961.2003.00517.x


Author Bekedam, Martijn A.
van Beek-Harmsen, Brechje J.
Boonstra, Anco
van Mechelen, Willem
Visser, Frans C.
van der Laarse, Willem J.
Title Maximum rate of oxygen consumption related to succinate dehydrogenase activity in skeletal muscle fibres of chronic heart failure patients and controls
Journal name Clinical Physiology and Functional Imaging   Check publisher's open access policy
ISSN 1475-0961
1475-097X
Publication date 2003-01-01
Sub-type Article (original research)
DOI 10.1046/j.1475-0961.2003.00517.x
Open Access Status
Volume 23
Issue 6
Start page 337
End page 343
Total pages 7
Place of publication Oxford
Publisher Blackwell
Language eng
Subject 1116 Medical Physiology
Abstract Previous studies indicate that the low maximum rate of oxygen consumption (VO2max) of chronic heart failure (CHF) patients is not because of impaired pump function of the heart. We hypothesize that VO2 during maximum exercise is determined by the total oxidative capacity of skeletal muscle. VO2max of six controls and 14 CHF patients, New York Heart Association class I–III, was determined using an incremental bicycle ergometer test. Cryostat sections of a biopsy from the quadriceps femoris muscle were incubated for succinate dehydrogenase (SDH) using quantitative histochemistry. VO2max (range: 29 ml O2 kg min−1 in a class III patient to 118 ml O2 kg min−1 in a control subject) correlates with the mean SDH activity of skeletal muscle fibres (r = 0·79 or r = 0·81, including or excluding oxygen uptake at rest, respectively; P<0·001). The relationship between VO2max and SDH activity is similar to that determined previously using isolated single muscle fibres and myocardial trabeculae under hyperoxic conditions. From the product of SDH activity and the cross-sectional area of the fibre (i.e. spatially integrated SDH activity), it is possible to calculate the maximum oxygen uptake rate per unit muscle fibre length. This uptake rate is linearly related to the number of capillaries per fibre (r = 0·76, P<0·001) in all subjects, suggesting that oxidative capacity of skeletal muscle fibres in CHF patients decreases in proportion to the oxygen supply capacity of the microcirculation
Keyword chronic heart failure
exercise
histochemistry
muscle biopsy
succinate dehydrogenase
VO2max
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Human Movement and Nutrition Sciences Publications
 
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