Poly(Adenosine Diphosphate-Ribose) Polymerase 1 Expression in Malignant Melanomas from Photoexposed Areas of the Head and Neck Region

Staibano, Stefania, Pepe, Stefano, Muzio, Lorenzo Lo, Somma, Pasquale, Mascolo, Massimo, Argenziano, Giuseppe, Scalvenzi, Massimiliano, Salvatore, Gaetano, Fabbrocini, Gabriella, Molea, Guido, Bianco, Angelo Raffaele, Carlomagno, Chiara and De Rosa, Gaetano (2005) Poly(Adenosine Diphosphate-Ribose) Polymerase 1 Expression in Malignant Melanomas from Photoexposed Areas of the Head and Neck Region. Human Pathology, 36 1: 724-731. doi:10.1016/j.humpath.2005.04.017


Author Staibano, Stefania
Pepe, Stefano
Muzio, Lorenzo Lo
Somma, Pasquale
Mascolo, Massimo
Argenziano, Giuseppe
Scalvenzi, Massimiliano
Salvatore, Gaetano
Fabbrocini, Gabriella
Molea, Guido
Bianco, Angelo Raffaele
Carlomagno, Chiara
De Rosa, Gaetano
Title Poly(Adenosine Diphosphate-Ribose) Polymerase 1 Expression in Malignant Melanomas from Photoexposed Areas of the Head and Neck Region
Journal name Human Pathology   Check publisher's open access policy
ISSN 0046-8177
1532-8392
Publication date 2005-07-01
Year available 2005
Sub-type Article (original research)
DOI 10.1016/j.humpath.2005.04.017
Open Access Status
Volume 36
Issue 1
Start page 724
End page 731
Total pages 8
Editor R. V. Lloyd
Place of publication United States
Publisher W. B. Saunders
Language eng
Subject 110304 Dermatology
Formatted abstract
The family of the poly(adenosine diphosphate-ribose) polymerase (PARP) proteins is directly involved in genomic stability, DNA repair, and apoptosis by DNA damage. In this study, we evaluated the role of PARP-1 in melanoma and its prognostic importance. We studied by immunohistochemistry and Western blot analysis PARP-1 expression in a selected series of 80 primary melanoma of the head and neck region. The results were correlated with tumor thickness and patient's outcome. A follow-up of at least 3 years was available. Fifteen cases of benign melanocytic nevi were used as controls. Normal melanocytes showed only scattered, focal nuclear positivity and were considered as negative for PARP-1 expression by immunohistochemistry (score, 0). Thirty cases of melanoma (37.5%) showed nuclear expression of PARP-1 in both radial and vertical growth phases. Western blot analysis showed the presence of a high signal for full-length PARP-1 only in the cases with high immunohistochemical (nuclear) expression of protein (score, ++/+++) in both radial and vertical growth phase. A significant correlation was present between PARP-1 expression in vertical growth phase and the thickness of tumor lesion (P = .014); all but one tumor measuring less than 0.75 mm showed no or low PARP-1 expression. No correlation was found between PARP-1 expression in radial growth phase and tumor thickness (P = .38, data not shown). These data suggest that PARP-1 overexpression is a potential novel molecular marker of aggressive cutaneous malignant melanoma and a direct correlation between PARP-1–mediated inhibition of the apoptosis and biologic behavior of cutaneous malignant melanoma.
Keyword Cutaneous melanoma
PARP-1
Prognosis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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Created: Fri, 03 Apr 2009, 20:50:27 EST by Mary-Anne Marrington on behalf of School of Medicine