Specific Response of a Novel and Abundant Lactobacillus amylovorus-Like Phylotype to Dietary Prebiotics in the Guts of Weaning Piglets

Konstantinov, Sergey R., Awati, Ajay, Smidt, Hauke, Williams, Barbara A., Akkermans, Antoon D. L. and de Vos, Willem M. (2004) Specific Response of a Novel and Abundant Lactobacillus amylovorus-Like Phylotype to Dietary Prebiotics in the Guts of Weaning Piglets. Applied and environmental microbiology, 70 7: 3821-3830. doi:10.1128/AEM.70.7.3821-3830.2004

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Author Konstantinov, Sergey R.
Awati, Ajay
Smidt, Hauke
Williams, Barbara A.
Akkermans, Antoon D. L.
de Vos, Willem M.
Title Specific Response of a Novel and Abundant Lactobacillus amylovorus-Like Phylotype to Dietary Prebiotics in the Guts of Weaning Piglets
Journal name Applied and environmental microbiology   Check publisher's open access policy
ISSN 0099-2240
1098-5336
Publication date 2004-07-01
Sub-type Article (original research)
DOI 10.1128/AEM.70.7.3821-3830.2004
Open Access Status File (Publisher version)
Volume 70
Issue 7
Start page 3821
End page 3830
Total pages 10
Place of publication Washington, D.C.
Publisher American Society for Microbiology
Language eng
Subject 070204 Animal Nutrition
Abstract Using 16S rRNA gene-based approaches, we analyzed the responses of ileal and colonic bacterial communities of weaning piglets to dietary addition of four fermentable carbohydrates (inulin, lactulose, wheat starch, and sugar beet pulp). An enriched diet and a control diet lacking these fermentable carbohydrates were fed to piglets for 4 days (n = 48), and 10 days (n = 48), and the lumen-associated microbiota were compared using denaturing gradient gel electrophoresis (DGGE) analysis of amplified 16S rRNA genes. Bacterial diversities in the ileal and colonic samples were measured by assessing the number of DGGE bands and the Shannon index of diversity. A higher number of DGGE bands in the colon (24.2 ± 5.5) than in the ileum (9.7 ± 4.2) was observed in all samples. In addition, significantly higher diversity, as measured by DGGE fingerprint analysis, was detected in the colonic microbial community of weaning piglets fed the fermentable-carbohydrate-enriched diet for 10 days than in the control. Selected samples from the ileal and colonic lumens were also investigated using fluorescent in situ hybridization (FISH) and cloning and sequencing of the 16S rRNA gene. This revealed a prevalence of Lactobacillus reuteri in the ileum and Lactobacillus amylovorus-like populations in the ileum and the colon in the piglets fed with fermentable carbohydrates. Newly developed oligonucleotide probes targeting these phylotypes allowed their rapid detection and quantification in the ileum and colon by FISH. The results indicate that addition of fermentable carbohydrates supports the growth of specific lactobacilli in the ilea and colons of weaning piglets.
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Centre for Nutrition and Food Sciences Publications
 
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Created: Fri, 03 Apr 2009, 18:19:29 EST by Ms Lynette Adams on behalf of Centre for Nutrition and Food Sciences