Antibody response against endogenous stages of an attenuated strain of Eimeria tenella

Constantinoiu, C. C., Molloy, J. B., Jorgensen, W. K. and Coleman, G. T. (2008) Antibody response against endogenous stages of an attenuated strain of Eimeria tenella. Veterinary Parasitology, 154 3-4: 193-204. doi:10.1016/j.vetpar.2008.03.029


Author Constantinoiu, C. C.
Molloy, J. B.
Jorgensen, W. K.
Coleman, G. T.
Title Antibody response against endogenous stages of an attenuated strain of Eimeria tenella
Formatted title
Antibody response against endogenous stages of anattenuated strain of Eimeria tenella
Journal name Veterinary Parasitology   Check publisher's open access policy
ISSN 0304-4017
Publication date 2008-07-04
Sub-type Article (original research)
DOI 10.1016/j.vetpar.2008.03.029
Open Access Status Not yet assessed
Volume 154
Issue 3-4
Start page 193
End page 204
Total pages 12
Editor L. S. Mansfield
C. Genchi
M. A. Taylor,
Place of publication Amsterdam, The Netherlands
Publisher Elsevier BV
Language eng
Subject C1
070708 Veterinary Parasitology
970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
Abstract The application of attenuated vaccines for the prevention of chicken coccidiosis has increased exponentially in recent years. In Eimeria infections, protective immunity is thought to rely on a strong cell mediated response with antibodies supposedly playing a minor role. However, under certain conditions antibodies seem to be significant in protection. Furthermore, antibodies could be useful for monitoring natural exposure of flocks to Eimeria spp. and for monitoring the infectivity of live vaccines. Our objective was to investigate the chicken antibody response to the different parasite lifecycle stages following infection with an attenuated strain of Eimeria tenella. Western blotting analysis of parasite antigens prepared from the lining of caeca infected with the attenuated strain of E. tenella revealed two dominant antigens of 32 and 34 kDa, apparently associated with trophozoites and merozoites that were present at high concentrations between 84 and 132 h post-infection. When cryosections of caeca infected with E. tenella were probed with IgY purified from immune birds the most intense reaction was observed with the asexual stages. Western blotting analysis of proteins of purified sporozoites and third generation merozoites and absorption of stage-specific antibodies from sera suggested that a large proportion of antigens is shared by the two stages. The time-courses of the antibody response to sporozoite and merozoite antigens were similar but varied depending on the inoculation regime and the degree of oocyst recirculation.
Keyword Eimeria tenella
Sporozoites
Merozoites
Antigen profiles
Antibody response
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Veterinary Science Publications
 
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Created: Fri, 03 Apr 2009, 01:26:19 EST by Narelle Poole on behalf of School of Veterinary Science