Analysis of ORF’s 2b, 3, 4, and partial ORF5 of sequential isolates of equine arteritis virus shows genetic variation following experimental infection of horses.

Liu, Lihong, Castillo-Olivares, Javier, Davis-Poynter, Nick J., Baule, Claudia, Xia, Hongyan and Belak, Sándor (2008) Analysis of ORF’s 2b, 3, 4, and partial ORF5 of sequential isolates of equine arteritis virus shows genetic variation following experimental infection of horses.. Veterinary Microbiology, 129 3-4: 262-268. doi:10.1016/j.vetmic.2007.11.021


Author Liu, Lihong
Castillo-Olivares, Javier
Davis-Poynter, Nick J.
Baule, Claudia
Xia, Hongyan
Belak, Sándor
Title Analysis of ORF’s 2b, 3, 4, and partial ORF5 of sequential isolates of equine arteritis virus shows genetic variation following experimental infection of horses.
Journal name Veterinary Microbiology   Check publisher's open access policy
ISSN 0378-1135
Publication date 2008-06-22
Year available 2008
Sub-type Article (original research)
DOI 10.1016/j.vetmic.2007.11.021
Open Access Status Not yet assessed
Volume 129
Issue 3-4
Start page 262
End page 268
Total pages 7
Editor U. Truyen
W. Gaastra
Place of publication Netherlands
Publisher Elsevier BV
Language eng
Subject C1
060506 Virology
060502 Infectious Agents
920109 Infectious Diseases
Abstract Samples from horses experimentally infected with the “large plaque variant (LP3A+)” of equine arteritis virus were analysed. These included 182 nasal swabs collected from day 1 to 14 post-infection (p.i.), and 21 virus isolates obtained from white blood cells of animals that showed a prolonged viraemia between days 30 to 72 p.i. In order to determine the genetic stability of the virus and particularly to characterise the genetic variants found during the prolonged viraemia, partial sequences of open reading frame 5 (ORF5) encoding glycoprotein 5 (GP5) were generated. Viruses with amino acid substitutions in GP5 were used for further amplification and sequencing of a fragment encompassing ORFs 2b, 3, and 4. The ORF5 nucleotide sequences of the virus present in 65 out of 66 nasal swabs were identical to that of the inoculated virus, suggesting that the ORF5 gene of LP3A+ was genetically stable during the first 2 weeks p.i. Contrary to this, a number of mutations were found in the ORF5 of virus isolates obtained from day 30 p.i. The mutations mainly clustered in antigenic neutralization site C within variable region 1 of the GP5 ectodomain. Sequence variability was also identified in ORFs 2b, 3 and 4, with ORF 4 having the highest proportion of non-synonymous changes (4/6).
Keyword Microbiology
Veterinary Sciences
Microbiology
Veterinary Sciences
MICROBIOLOGY
VETERINARY SCIENCES
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
Clinical Medical Virology Centre Publications
 
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Created: Thu, 02 Apr 2009, 23:25:25 EST by Lesley Arnicar on behalf of Clinical Medical Virology Centre