The antimicrobial effect of heparin on common respiratory pathogens

Zappala, Christopher, Chandan, Snehal, George, Narelle, Faoagali, Joan and Boots, Robert J. (2007) The antimicrobial effect of heparin on common respiratory pathogens. Critical Care and Resuscitation Journal, 9 2: 157-160.

Author Zappala, Christopher
Chandan, Snehal
George, Narelle
Faoagali, Joan
Boots, Robert J.
Title The antimicrobial effect of heparin on common respiratory pathogens
Journal name Critical Care and Resuscitation Journal   Check publisher's open access policy
ISSN 1441-2772
Publication date 2007-06-01
Year available 2007
Sub-type Article (original research)
Open Access Status Not Open Access
Volume 9
Issue 2
Start page 157
End page 160
Total pages 4
Editor Rinaldo Bellomo
Place of publication Melbourne, VIC, Australia
Publisher Australasian Academy of Critical Care Medicin
Language eng
Subject 110802 Medical Infection Agents (incl. Prions)
110310 Intensive Care
Formatted abstract
Aim: The mucolytic, anticoagulative, anti-inflammatory and neo-angiogenic properties of inhaled heparin may benefit patients with burns and cystic fibrosis. We assessed the antibacterial effects of unfractionated heparin.

Methods: Stored clinical isolates of Acinetobacter baumannii (n= 4), Candida albicans (n = 5), Haemophilus influenzae (n= 5), Klebsiella pneumoniae (n = 4), methicillin-resistant Staphylococcus aureus (n= 3), Pseudomonas aeruginosa (n = 2), and Streptococcus pneumoniae (n = 7) were subcultured on horse blood agar, incubated at 35°C overnight, then inoculated into trypticase soy broth to a density of 1 McFarland standard. Dilutions of unfractionated heparin (containing 250– 7500 U) and 100 μL of the 1.0 McFarland standard broth were incubated at 35°C overnight in microtitre plates and then subcultured on horse blood agar using 1 μL standard loops. Colonies (representing viable organisms) were counted.

Results: Heparin produced dose-dependent growth inhibition of three of seven S. pneumoniae isolates (complete inhibition at 2500 U dose per 200 μL) and one of five H. influenzae isolates (complete inhibition at 7500U dose per 200 μL), but no inhibition of other isolates.

Conclusions: Unfractionated heparin is unlikely to have antibacterial effects because of its unpredictable inhibition of growth of common respiratory pathogens.
Q-Index Code C1
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
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Created: Thu, 02 Apr 2009, 23:21:00 EST by Mary-Anne Marrington on behalf of Anaesthesiology and Critical Care - RBWH