Differences in microbial activity of digesta from three sections of the porcine large intestine according to in vitro fermentation of carbohydrate-rich substrates

Bauer, Eva, Williams, Barbara A., Bosch, Marlou W., Voigt, Christina, Mosenthin, Rainer and Verstegen, Martin W. A . (2004) Differences in microbial activity of digesta from three sections of the porcine large intestine according to in vitro fermentation of carbohydrate-rich substrates. Journal of the science of food and agriculture, 84 15: 2097-2104. doi:10.1002/jsfa.1845


Author Bauer, Eva
Williams, Barbara A.
Bosch, Marlou W.
Voigt, Christina
Mosenthin, Rainer
Verstegen, Martin W. A .
Title Differences in microbial activity of digesta from three sections of the porcine large intestine according to in vitro fermentation of carbohydrate-rich substrates
Journal name Journal of the science of food and agriculture   Check publisher's open access policy
ISSN 1097-0010
0022-5142
Publication date 2004-12-01
Sub-type Article (original research)
DOI 10.1002/jsfa.1845
Open Access Status Not yet assessed
Volume 84
Issue 15
Start page 2097
End page 2104
Total pages 8
Place of publication Chichester, England
Publisher John Wiley & Sons on behalf of SCI
Language eng
Subject 070204 Animal Nutrition
Abstract To determine whether faecal microorganisms can represent the entire large intestinal population, samples from caecum, mid-colon and rectum of three adult pigs were used for the in vitro fermentation of fructo-oligosaccharide (FOS), potato starch, wheat bran and oat hulls. The cumulative gas production technique measured fermentation kinetics and end-products such as total gas, NH3 and volatile fatty acids (VFA). There were significant differences in the fermentability of substrates, in terms of both kinetics and end-products. More relevant to this study, there were also differences between pigs in respect of total gas production, the rate of gas production (RM) and VFA production. For large intestine sections, there were more VFA from mid-colon and rectal inocula compared with that from the caecum (p < 0.0001). Total gas, RM and NH3 were highest for rectal, intermediate for mid-colon and lowest for caecal inocula (p < 0.0001). It was concluded that, while faecal sampling might overestimate caecal fermentation, its use is valid for in vitro assessment of large intestinal fermentation. However, differences between pigs indicate that a mix of samples from several animals remains important. Copyright © 2004 Society of Chemical Industry
Keyword carbohydrates
digesta
fermentation kinetics
in vitro
large intestine
pig
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Centre for Nutrition and Food Sciences Publications
 
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Created: Thu, 02 Apr 2009, 23:15:04 EST by Ms Lynette Adams on behalf of Centre for Nutrition and Food Sciences