Analysis of 16S rDNA reveals bacterial shift during in vitro fermentation of fermentable carbohydrate using piglet faeces as inoculum

Zhu, Wei-Yun, Williams, Barbara A., Konstantinov, Sergey R., Tamminga, Seerp, De Vos, Willem M. and Akkermans, Antoon D. L. (2003) Analysis of 16S rDNA reveals bacterial shift during in vitro fermentation of fermentable carbohydrate using piglet faeces as inoculum. Anaerobe, 9 4: 175-180. doi:10.1016/S1075-9964(03)00083-0


Author Zhu, Wei-Yun
Williams, Barbara A.
Konstantinov, Sergey R.
Tamminga, Seerp
De Vos, Willem M.
Akkermans, Antoon D. L.
Title Analysis of 16S rDNA reveals bacterial shift during in vitro fermentation of fermentable carbohydrate using piglet faeces as inoculum
Journal name Anaerobe   Check publisher's open access policy
ISSN 1075-9964
Publication date 2003-08-01
Sub-type Article (original research)
DOI 10.1016/S1075-9964(03)00083-0
Volume 9
Issue 4
Start page 175
End page 180
Total pages 6
Place of publication London ; San Diego
Publisher Academic Press
Language eng
Subject 070204 Animal Nutrition
Abstract In vitro fermentation of sugar beet pulp (SBP) was carried out to determine which bacterial species would be enriched by use of this carbohydrate source. Faeces from four weaning piglets as a source of inoculum was also compared. The microbial diversity of the prominent bacteria before and after this in vitro fermentation was analysed using denaturing gradient gel electrophoresis (DGGE) of PCR amplicons of 16S rDNA. Before fermentation, the DGGE profiles showed differences between cultures inoculated with faeces from different piglets, though some bands were common to all piglets. After fermentation of SBP, three dominant bands appeared, of which two bands appeared in all samples and one for both replicates of one piglet. Sequences of the corresponding 16S rDNA of two bands showed 92% similarity to Eubacterium eligens and 96% similarity to Lachnospira pectinoschiza, and that of the third band 95% to L. pectinoschiza.
Keyword In vitro fermentation
16S rDNA analysis
Prebiotics
Lachnospira pectinoschiza
Eubacterium eligens
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Centre for Nutrition and Food Sciences Publications
 
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Created: Thu, 02 Apr 2009, 21:23:06 EST by Ms Lynette Adams on behalf of Centre for Nutrition and Food Sciences