Effect of fermentable carbohydrates on piglet faecal bacterial communities as revealed by denaturing gradient gel electrophoresis analysis of 16S ribosomal DNA

Konstantinov, Sergey R., Zhu, Wei-Yun, Williams, Barbara A., Tamminga, Seerp, de Vos, Willem M. and Akkermans, Antoon D. L. (2003) Effect of fermentable carbohydrates on piglet faecal bacterial communities as revealed by denaturing gradient gel electrophoresis analysis of 16S ribosomal DNA. FEMS microbiology ecology, 43 2: 225-235. doi:10.1016/S0168-6496(02)00430-0


Author Konstantinov, Sergey R.
Zhu, Wei-Yun
Williams, Barbara A.
Tamminga, Seerp
de Vos, Willem M.
Akkermans, Antoon D. L.
Title Effect of fermentable carbohydrates on piglet faecal bacterial communities as revealed by denaturing gradient gel electrophoresis analysis of 16S ribosomal DNA
Journal name FEMS microbiology ecology   Check publisher's open access policy
ISSN 0168-6496
1574-6941
Publication date 2003-03-01
Sub-type Article (original research)
DOI 10.1016/S0168-6496(02)00430-0
Open Access Status DOI
Volume 43
Issue 2
Start page 225
End page 235
Total pages 11
Place of publication Oxford, England
Publisher Blackwell
Language eng
Subject 070204 Animal Nutrition
Abstract The effect of fermentable carbohydrates (sugar beet pulp and fructooligosaccharides) on the faecal bacterial communities of weaning piglets was analysed using 16S rDNA-based approaches. Amplicons of the V6–V8 variable regions of bacterial 16S rDNA were analysed by denaturing gradient gel electrophoresis (DGGE), cloning and sequencing. Differences in piglet faecal bacterial community structure were determined based on the Dice coefficients for pairwise comparison of the DGGE fingerprints and revealed significant changes in the faecal microbiota immediately after weaning. Piglets fed with fermentable carbohydrates showed a higher bacterial diversity and a more rapid stabilisation of the bacterial community compared with that of the animals fed with the control diet. Thirteen dominant DGGE bands were matched with sequences that showed 91–97% similarity to those derived from the Clostridium coccoides group and the Clostridium leptum subgroup. Amplicons related to Ruminococcus-like species were found in all DGGE fingerprints derived from pigs on the diet containing sugar beet pulp and fructooligosaccharides, but not in pigs on the control diet. These results indicate that these bacteria may play a role in the utilisation of dietary fibres.
Keyword Bacterial diversity
16S rDNA
Denaturing gradient gel electrophoresis
Weaning piglets
Sugar beet pulp and fructooligosaccharides
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Centre for Nutrition and Food Sciences Publications
 
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Created: Thu, 02 Apr 2009, 21:12:25 EST by Ms Lynette Adams on behalf of Centre for Nutrition and Food Sciences