Field fertility of sex-sorted and non-sorted frozen-thawed stallion spermatozoa

J.R. Clulow, H. Buss, H. Sieme, J.A. Rodger, A.J. Cawdell-Smith, G. Evans, D. Rath, L.H.A. Morris and W.M.C. Maxwell (2008) Field fertility of sex-sorted and non-sorted frozen-thawed stallion spermatozoa. Animal Reproduction Science, 108 3-4: 287-297. doi:10.1016/j.anireprosci.2007.08.015

Author J.R. Clulow
H. Buss
H. Sieme
J.A. Rodger
A.J. Cawdell-Smith
G. Evans
D. Rath
L.H.A. Morris
W.M.C. Maxwell
Title Field fertility of sex-sorted and non-sorted frozen-thawed stallion spermatozoa
Journal name Animal Reproduction Science   Check publisher's open access policy
ISSN 0378-4320
Publication date 2008-01-01
Year available 2007
Sub-type Article (original research)
DOI 10.1016/j.anireprosci.2007.08.015
Open Access Status
Volume 108
Issue 3-4
Start page 287
End page 297
Total pages 11
Editor A C O Evans
G N Hinch
J E Kinder
Place of publication Netherlands
Publisher Elseveir BV
Language eng
Subject C1
070206 Animal Reproduction
830306 Horses
Abstract In the 2004/2005 breeding season, the fertility of sex-sorted (SS) and non-sorted (NS) frozen stallion spermatozoa from two Hannovarian stallions was compared. A hysteroscopic insemination technique [Morris, L.H., Tiplady, C., Allen, W.R., 2003a. Pregnancy rates in mares after a single fixed time hysteroscopic insemination of low numbers of frozen-thawed spermatozoa onto the uterotubal junction. Equine Vet. J. 35, 197-201] was used to deposit low doses (6, 13 or 25 x 10(6) frozen-thawed SS or NS spermatozoa) onto the utero-tubal junction at 32 or 38 h after the administration of Chorulon (2500 IU, Intervet). Fertility was low, with one pregnancy (13 x 10(6) Spermatozoa, 500 mu L) obtained after artificial insemination with frozen SS spermatozoa (n=29 cycles) which resulted in the birth of a filly. Two pregnancies were obtained in mares inseminated with 6 x 10(6) NS spermatozoa in 250 mu L (n= 31 cycles). Mares failing to conceive on two experimental cycles were allocated to the conventional insemination group. Insemination with >500 x 10(6) motile NS frozen-thawed spermatozoa, yielded satisfactory per cycle conception rates (35.5%, 22/62) for both stallions combined and was within the values of their normal fertility as quoted by the stud's records. This suggests that the quality of the frozen semen was acceptable and that the freezing processes yielded viable spermatozoa capable of fertilisation. The poor fertility after hysteroscopic insemination with low doses of sex-sorted or non-sorted spermatozoa from the same stallions may be directly attributable to the low dose insemination conditions with frozen-thawed rather than sex-sorted spermatozoa. (C) 2007 Elsevier B.V. All rights reserved.
Keyword Agriculture, Dairy & Animal Science
Reproductive Biology
Reproductive Biology
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Epublication 16th September 2007, published in print November 2008

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 19 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 21 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 02 Apr 2009, 20:43:28 EST by Mrs Kathy Bachmann on behalf of School of Animal Studies