Cytoskeletal Tropomyosin Tm5NM1 Is Required for Normal Excitation–Contraction Coupling in Skeletal Muscle

Vlahovich, Nicole, Kee, Anthony J., Van der Pool, Chris, Kettle, Emma, Hernandez-Deviez, Delia, Lucas, Christine, Lynch, Gordon S., Parton, Robert G., Gunning, Peter W. and Hardeman, Edna C. (2009) Cytoskeletal Tropomyosin Tm5NM1 Is Required for Normal Excitation–Contraction Coupling in Skeletal Muscle. Molecular biology of the cell, 20 1: 400-409. doi:10.1091/mbc.E08-06-0616

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Author Vlahovich, Nicole
Kee, Anthony J.
Van der Pool, Chris
Kettle, Emma
Hernandez-Deviez, Delia
Lucas, Christine
Lynch, Gordon S.
Parton, Robert G.
Gunning, Peter W.
Hardeman, Edna C.
Title Cytoskeletal Tropomyosin Tm5NM1 Is Required for Normal Excitation–Contraction Coupling in Skeletal Muscle
Journal name Molecular biology of the cell   Check publisher's open access policy
ISSN 1059-1524
Publication date 2009-01-01
Year available 2009
Sub-type Article (original research)
DOI 10.1091/mbc.E08-06-0616
Open Access Status File (Publisher version)
Volume 20
Issue 1
Start page 400
End page 409
Total pages 9
Place of publication Bethesda, USA
Publisher American Society Biology
Language eng
Subject C1
060110 Receptors and Membrane Biology
970106 Expanding Knowledge in the Biological Sciences
Abstract The functional diversity of the actin microfilaments relies in part on the actin binding protein tropomyosin (Tm). The muscle-specific Tms regulate actin-myosin interactions and hence contraction. However, there is less known about the roles of the numerous cytoskeletal isoforms. We have shown previously that a cytoskeletal Tm, Tm5NM1, defines a Z-line adjacent cytoskeleton in skeletal muscle. Recently, we identified a second cytoskeletal Tm in this region, Tm4. Here we show that Tm4 and Tm5NM1 define separate actin filaments; the former associated with the terminal sarcoplasmic reticulum (SR) and other tubulovesicular structures. In skeletal muscles of Tm5NM1 knockout (KO) mice, Tm4 localization was unchanged, demonstrating the specificity of the membrane association. Tm5NM1 KO muscles exhibit potentiation of T-system depolarization and decreased force rundown with repeated T-tubule depolarizations consistent with altered T-tubule function. These results indicate that a Tm5NM1-defined actin cytoskeleton is required for the normal excitation–contraction coupling in skeletal muscle.
Keyword Cell Biology
Cell Biology
CELL BIOLOGY
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 321705
163626
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: 2009 Higher Education Research Data Collection
 
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Created: Thu, 02 Apr 2009, 02:33:50 EST by Cody Mudgway on behalf of Institute for Molecular Bioscience