Post-natal development of the porcine microbiota composition and activities

Konstantinov, Sergey R., Awati, Ajay A., Williams, Barbara A., Miller, Bevis G., Jones, Philip, Stokes, Christopher R., Akkermans, Antoon D. L., Smidt, Hauke and de Vos, Willem M. (2006) Post-natal development of the porcine microbiota composition and activities. Environmental Microbiology, 8 7: 1191-1199. doi:10.1111/j.1462-2920.2006.01009.x


Author Konstantinov, Sergey R.
Awati, Ajay A.
Williams, Barbara A.
Miller, Bevis G.
Jones, Philip
Stokes, Christopher R.
Akkermans, Antoon D. L.
Smidt, Hauke
de Vos, Willem M.
Title Post-natal development of the porcine microbiota composition and activities
Journal name Environmental Microbiology   Check publisher's open access policy
ISSN 1462-2912
1462-2920
Publication date 2006-07-01
Sub-type Article (original research)
DOI 10.1111/j.1462-2920.2006.01009.x
Open Access Status DOI
Volume 8
Issue 7
Start page 1191
End page 1199
Total pages 9
Place of publication Oxford
Publisher Blackwell Science
Language eng
Subject 070204 Animal Nutrition
Formatted abstract
The current study describes the development of the porcine microbiota and its metabolic activities during the neonatal and weaning period. Using 16S rRNA-based approaches, we first analysed the ileal and colonic microbiota of neonatal piglets at days 2, 5 and 12 after birth. To further investigate the effect of weaning at 3 weeks of age, 19-day-old piglets (n = 64) were randomly allocated into two groups. Half of the piglets remained with their sows throughout the study, while the remaining piglets were weaned. As revealed by sequence analysis of 16S rRNA gene amplicons, the samples of 2-day-old piglets harboured a consortium of bacteria related to Escherichia coli, Shigella flexneri, Lactobacillus sobrius, Lactobacillus reuteri and Lactobacillus acidophilus. Moreover, species-specific real-time polymerase chain reaction assays unveiled that L. sobrius and L. reuteri predominated in the ileal samples of the neonatal and unweaned piglets with population levels up to 7 × 108 cells per gram of lumen content. Following weaning, however, these two lactobacilli were detected at significantly lower levels (< 103) in the ileal samples. Furthermore, a shift in composition and metabolic activities of the predominant microbiota, and emergence of clostridia and E. coli, were encountered in the intestinal samples of the piglets after the early post-weaning period.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Nutrition and Food Sciences Publications
 
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Created: Thu, 02 Apr 2009, 00:41:52 EST by Alexandra Cooney on behalf of Centre for Nutrition and Food Sciences