Bombyx mori Silk Fibroin Membranes as Potential substrata for epithelial constructs used in the management of ocular surface disorders

Chirila, Traian V., Barnard, Zeke, Zainuddin, Harkin, Damien G., Schwab, Ivan R. and Hirst, Lawrence W. (2008) Bombyx mori Silk Fibroin Membranes as Potential substrata for epithelial constructs used in the management of ocular surface disorders. Tissue Engineering Part A, 14 7: 1203-1211. doi:10.1089/ten.tea.2007.0224


Author Chirila, Traian V.
Barnard, Zeke
Zainuddin
Harkin, Damien G.
Schwab, Ivan R.
Hirst, Lawrence W.
Title Bombyx mori Silk Fibroin Membranes as Potential substrata for epithelial constructs used in the management of ocular surface disorders
Formatted title
Bombyx mori Silk Fibroin Membranes as Potential substrata for epithelial constructs used in the management of ocular surface disorders
Journal name Tissue Engineering Part A   Check publisher's open access policy
ISSN 1937-3341
Publication date 2008-07-01
Year available 2008
Sub-type Article (original research)
DOI 10.1089/ten.tea.2007.0224
Open Access Status
Volume 14
Issue 7
Start page 1203
End page 1211
Total pages 9
Editor Antonios G. Mikos
Peter C. Johnson
Place of publication New Rochelle, NY
Publisher Mary Ann Liebert Inc
Language eng
Subject C1
970111 Expanding Knowledge in the Medical and Health Sciences
111301 Ophthalmology
Abstract Membranes were prepared from fibroin, a protein isolated from the domesticated silkworm (Bombyx mori) silk, and evaluated as a potential substratum for corneal limbal epithelial cells. These membranes (i.e., B. mori silk fibroin [BMSF] membranes) were cast from dialyzed solutions of fibroin protein (4% w/v) dispensed into 35-mm-diameter culture dishes and dried at room temperature (23–24°C). The resulting material was transparent, easy to handle, and supported levels of human limbal epithelial (HLE) cell growth comparable to that observed on tissue culture plastic. Remarkably, these results were obtained utilizing a commercial serum-free medium (CnT-20) designed for the ex vivo expansion of corneal epithelial progenitor cells. The potential benefits of serum proteins on this culture system were examined through addition of fetal bovine serum (FBS) either to fibroin stocks prior to membrane casting or by supplementation of the CnT-20 medium. Membranes cast in the presence of FBS displayed increasing opacity and induced little change in HLE growth. Supplementation of CnT-20 medium with FBS deterred cell growth on all substrata, including tissue culture plastic control substrata. The remarkable properties of BMSF membranes demonstrated under serum-free conditions warrant investigation of this material as a substratum in the creation of tissue-engineered constructs for the restoration of diseased or damaged ocular surface.
Keyword Ocular Surface Disorders
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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Created: Thu, 02 Apr 2009, 00:39:23 EST by Kanthi Wijesoma on behalf of School of Medicine