Synthesis and characterization of alginate/poly-L-ornithine/alginate microcapsules for local immunosuppression

Leung, A.H.M., Lawrie, G.A., Nielsen, L.K. and Trau, M. (2008) Synthesis and characterization of alginate/poly-L-ornithine/alginate microcapsules for local immunosuppression. Journal of Microencapsulation, 25 6: 387-398. doi:10.1080/02652040802008857


Author Leung, A.H.M.
Lawrie, G.A.
Nielsen, L.K.
Trau, M.
Title Synthesis and characterization of alginate/poly-L-ornithine/alginate microcapsules for local immunosuppression
Journal name Journal of Microencapsulation   Check publisher's open access policy
ISSN 0265-2048
Publication date 2008-01-01
Year available 2008
Sub-type Article (original research)
DOI 10.1080/02652040802008857
Open Access Status Not yet assessed
Volume 25
Issue 6
Start page 387
End page 398
Total pages 12
Place of publication United Kingdom
Publisher Informa Healthcare
Language eng
Subject C1
090301 Biomaterials
970103 Expanding Knowledge in the Chemical Sciences
Abstract Alginate/poly-L-ornithine/alginate (APA) coherent microencapsulation, which provides an immunoselective and highly biocompatible membrane, creates a viable option for cellular or tissue transplantation. This study explored the potential of incorporating immunosuppressive drugs onto the capsule surface to provide local immunosuppression in addition to immunoisolation. A thorough investigation has been conducted to optimize and characterize alginate biotinylation via carbodiimide chemistry by a 4'-hydroxyazobenzene-2-carboxylic acid (HABA) based assay and by ATR-FTIR, H-NMR and XPS. To minimize the formation of by-product, a theoretical 40% activation of the carboxylic group on the alginate was employed to manufacture an optimal modification of similar to 10% biotinylated alginate. Confocal fluorescence microscopy was used to assess the conjugation of streptavidin and assembly of antibodies on the microcapsules. Local immunosuppressive capacity was assimilated on the APA microcapsules by binding of anti-tumour necrosis factor-alpha (TNF-alpha) antibodies via streptavidin-biotin conjugation, shown from the clear reduction of TNF-alpha in in-vitro medium.
Keyword alginate
microencapsulation
cross-linking
surface modification
Immunomodulation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 15 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 19 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 01 Apr 2009, 20:07:42 EST by Jennifer Falknau on behalf of School of Chemistry & Molecular Biosciences