A highly structured,nuclease-resistant, noncoding RNA produced by flaviviruses is required for pathogenicity

Pijlman, Gorben P., Funk, Anneke, Kondratieva, Natasha, Leung, Jason, Torres, Shessy, Van der Aa, Lieke, Liu, Wen Jun, Palmenberg, Ann C., Shi, Pei-Yong, Hall, Roy A. and Khromykh, Alexander A. (2008) A highly structured,nuclease-resistant, noncoding RNA produced by flaviviruses is required for pathogenicity. Cell Host and Microbe, 4 6: 579-591. doi:10.1016/j.chom.2008.10.007


Author Pijlman, Gorben P.
Funk, Anneke
Kondratieva, Natasha
Leung, Jason
Torres, Shessy
Van der Aa, Lieke
Liu, Wen Jun
Palmenberg, Ann C.
Shi, Pei-Yong
Hall, Roy A.
Khromykh, Alexander A.
Title A highly structured,nuclease-resistant, noncoding RNA produced by flaviviruses is required for pathogenicity
Journal name Cell Host and Microbe   Check publisher's open access policy
ISSN 1931-3128
Publication date 2008-12-11
Year available 2008
Sub-type Article (original research)
DOI 10.1016/j.chom.2008.10.007
Open Access Status Not yet assessed
Volume 4
Issue 6
Start page 579
End page 591
Total pages 13
Place of publication Cambridge, Mass., United States
Publisher Cell Press
Language eng
Subject C1
030307 Theory and Design of Materials
060502 Infectious Agents
060506 Virology
920109 Infectious Diseases
970106 Expanding Knowledge in the Biological Sciences
970111 Expanding Knowledge in the Medical and Health Sciences
Abstract Viral noncoding RNAs have been shown to play an important role in virus-host interplay to facilitate virus replication. We report that members of the genus Flavivirus, a large group of medically important encephalitic RNA viruses, produce a unique and highly structured noncoding RNA of 0.3–0.5 kb derived from the 3′ untranslated region of the viral genome. Using West Nile virus as a model, we show that this subgenomic RNA is a product of incomplete degradation of viral genomic RNA by cellular ribonucleases. Highly conserved RNA structures located at the beginning of the 3′ untranslated region render this RNA resistant to nucleases, and the resulting subgenomic RNA product is essential for virus-induced cytopathicity and pathogenicity. Thus, flaviviruses evolved a unique strategy to generate a noncoding RNA product that allows them to kill the host more efficiently.
Keyword WEST-NILE-VIRUS
UNFOLDED PROTEIN RESPONSE
KUNJIN VIRUS
SECONDARY STRUCTURE
ENCEPHALITIS-VIRUS
SACCHAROMYCES-CEREVISIAE
CYCLIZATION SEQUENCES
UNTRANSLATED REGION
GENETIC ALGORITHM
Apoptotic Pathways
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
 
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Created: Wed, 01 Apr 2009, 18:27:24 EST by Jennifer Falknau on behalf of School of Chemistry & Molecular Biosciences