Microbial diversity studies of the porcine gastrointestinal ecosystem during weaning transition

Konstantinov, Sergey R., Favier, Christine F., Zhu, Wei Yun, Williams, Barbara A., Klüß, Jeannette, Souffrant, Wolfgang-Bernhard, de Vos, Willem M., Akkermans, Antoon D.L. and Smidt, Hauke (2004) Microbial diversity studies of the porcine gastrointestinal ecosystem during weaning transition. Animal Research, 53 4: 317-324. doi:10.1051/animres:2004019

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Author Konstantinov, Sergey R.
Favier, Christine F.
Zhu, Wei Yun
Williams, Barbara A.
Klüß, Jeannette
Souffrant, Wolfgang-Bernhard
de Vos, Willem M.
Akkermans, Antoon D.L.
Smidt, Hauke
Title Microbial diversity studies of the porcine gastrointestinal ecosystem during weaning transition
Journal name Animal Research   Check publisher's open access policy
ISSN 1627-3583
Publication date 2004-01-01
Sub-type Article (original research)
DOI 10.1051/animres:2004019
Open Access Status File (Publisher version)
Volume 53
Issue 4
Start page 317
End page 324
Total pages 8
Publisher INRA, EDP Sciences
Language eng
Subject 070204 Animal Nutrition
Abstract At the time of weaning, major quantitative and qualitative changes occur in the composition of the intestinal microbiota of piglets, influenced by diet, environmental factors, and the host. Within a short period of time, the intestinal microbiota must ultimately develop from a simple, unstable community into a complex and stable one. Here we present data on the development of the intestinal microbiota based on 16S rRNA gene sequence diversity. In addition to a PCR-based analysis of the 16S rRNA gene by cloning and denaturing gradient gel electrophoresis (DGGE), data on fluorescent in situ hybridisation (FISH) are presented to quantify the total bacterial communities, major Lactobacillus populations and specific Lactobacillus species. The results reported here indicate that the addition of non-digestible, fermentable carbohydrates (= prebiotics) leads to an enrichment of lactobacilli in the small intestine, and increased stability and diversity of the bacterial community in the colon. The data support the hypothesis that changes of the diet can modulate the composition of the microbiota in the intestine. These findings may have potentially major implications for the development of dietary strategies aiming to improve animal health during the weaning process.
Keyword 16S rRNA gene
Pig GI tract microbiota
Q-Index Code C1
Additional Notes Review Article

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Centre for Nutrition and Food Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 59 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 01 Apr 2009, 00:20:31 EST by Ms Sarada Rao on behalf of Centre for Nutrition and Food Sciences