Dimethylacetamide can be used as an alternative to glycerol for the successful cryopreservation of koala (Phascolarctos cinereus) spermatozoa

Zee, Yeng Peng, Holt, William V., Gosalvez, Jaime, Allen, Camryn D., Nicolson, Vere, Pyne, Michael, Burridge, Michelle, Carrick, Frank N. and Johnston, Stephen D. (2008) Dimethylacetamide can be used as an alternative to glycerol for the successful cryopreservation of koala (Phascolarctos cinereus) spermatozoa. Reproduction Fertility and Development, 20 6: 724-733. doi:10.1071/RD08036


Author Zee, Yeng Peng
Holt, William V.
Gosalvez, Jaime
Allen, Camryn D.
Nicolson, Vere
Pyne, Michael
Burridge, Michelle
Carrick, Frank N.
Johnston, Stephen D.
Title Dimethylacetamide can be used as an alternative to glycerol for the successful cryopreservation of koala (Phascolarctos cinereus) spermatozoa
Journal name Reproduction Fertility and Development   Check publisher's open access policy
ISSN 1031-3613
1448-5990
Publication date 2008-07-01
Sub-type Article (original research)
DOI 10.1071/RD08036
Open Access Status
Volume 20
Issue 6
Start page 724
End page 733
Total pages 10
Editor Sharon Mortimer
Place of publication Melbourne, Australia
Publisher C S I R O Publishing
Language eng
Subject C1
060602 Animal Physiology - Cell
970106 Expanding Knowledge in the Biological Sciences
Abstract Swelling of koala sperm chromatin following cryopreservation has largely been attributed to the absence of intermolecular disulfide cross-linkages in the marsupial sperm nucleus. Fish spermatozoa also lack disulfide bonds within their chromatin, but have been successfully cryopreserved. The present study examined the hypothesis that the cryoprotectants used for fish sperm cryopreservation would confer a similar degree of protection on koala spermatozoa. Three concentrations each of five cryoprotectants (dimethyl sulfoxide, methanol, propylene glycol, ethylene glycol and dimethylacetamide (DMA)) were evaluated. Each treatment was compared against an established koala sperm cryopreservation protocol that uses 14% glycerol. Post-thaw assessment of progressive motility, plasma membrane integrity and mitochondrial membrane potential (MMP) revealed that protocols using 15% DMA achieved 62.2 ± 3.6% (P < 0.05) sperm survival, of which 79% (P < 0.05) had high MMP, an improvement of 32% and 40%, respectively, over sperm frozen in 14% glycerol. The percentage of spermatozoa with swollen nuclei was also lowest when frozen in 15% DMA, both immediately after thawing (18.0 ± 3.5%; P < 0.05) and after 2 h incubation at 35°C (35.8 ± 4.4%; P < 0.05). A second study was conducted to determine the optimal concentration of DMA for use in the cryopreservation of koala spermatozoa. High DMA concentrations (17.5% and 20%) resulted in significantly lower proportions of live spermatozoa showing high MMP immediately after thawing compared with spermatozoa frozen in the lower concentrations. The percentage of koala spermatozoa with swollen chromatin following cryopreservation was not affected by DMA concentration. © CSIRO 2008
Keyword Amides
JC-1
Mitochondrial function
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
 
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Created: Tue, 31 Mar 2009, 21:31:47 EST by Mrs Kathy Bachmann on behalf of School of Animal Studies