Cryopreservation of kangaroo spermatozoa using alternative approaches that reduce cycotoxic exposure to glycerol

McClean, Rhett, Zee, Yeng Peng, Holt, William V. and Johnston, Stephen D. (2008) Cryopreservation of kangaroo spermatozoa using alternative approaches that reduce cycotoxic exposure to glycerol. Cryobiology, 57 3: 304-307. doi:10.1016/j.cryobiol.2008.08.007


Author McClean, Rhett
Zee, Yeng Peng
Holt, William V.
Johnston, Stephen D.
Title Cryopreservation of kangaroo spermatozoa using alternative approaches that reduce cycotoxic exposure to glycerol
Journal name Cryobiology   Check publisher's open access policy
ISSN 0011-2240
1090-2392
Publication date 2008-12-01
Sub-type Article (original research)
DOI 10.1016/j.cryobiol.2008.08.007
Open Access Status DOI
Volume 57
Issue 3
Start page 304
End page 307
Total pages 4
Editor David E. Pegg
Place of publication Rockville, MD., U.S.A.
Publisher Academic Press
Language eng
Subject C1
060602 Animal Physiology - Cell
970106 Expanding Knowledge in the Biological Sciences
0601 Biochemistry and Cell Biology
1116 Medical Physiology
Abstract Alternative techniques for the cryopreservation of kangaroo spermatozoa that reduced or eliminated the need for glycerol were investigated including; (1) freezing spermatozoa with 20% glycerol in pre-packaged 0.25 mL Cassou straws to enable rapid dilution of the glycerol post-thaw, (2) investigating the efficacy of 20% (v/v) dimethyl sulphoxide (DMSO) and dimethylacetamide (DMA-10%, 15% and 20% v/v) as cryoprotectants and (3) vitrification of spermatozoa with or without cryoprotectant (20% v/v glycerol, 20% v/v DMSO and 20% v/v DMA). Immediate in-straw post-thaw dilution of 20% glycerol and cryopreservation of spermatozoa in 20% DMSO produced no significant improvement in post-thaw viability of kangaroo spermatozoa. Spermatozoa frozen in 20% DMA showed post-thaw motility and plasma membrane integrity of 12.7+/-1.9% and 22.7+/-5.4%, respectively, while kangaroo spermatozoa frozen by ultra-rapid freezing techniques showed no evidence of post-thaw viability. The use of 10-20% DMA represents a modest but significant improvement in the development of a sperm cryopreservation procedure for kangaroos.
Formatted abstract
Alternative techniques for the cryopreservation of kangaroo spermatozoa that reduced or eliminated the need for glycerol were investigated including; (1) freezing spermatozoa with 20% glycerol in pre-packaged 0.25 mL Cassou straws to enable rapid dilution of the glycerol post-thaw, (2) investigating the efficacy of 20% (v/v) dimethyl sulphoxide (DMSO) and dimethylacetamide (DMA—10%, 15% and 20% v/v) as cryoprotectants and (3) vitrification of spermatozoa with or without cryoprotectant (20% v/v glycerol, 20% v/v DMSO and 20% v/v DMA). Immediate in-straw post-thaw dilution of 20% glycerol and cryopreservation of spermatozoa in 20% DMSO produced no significant improvement in post-thaw viability of kangaroo spermatozoa. Spermatozoa frozen in 20% DMA showed post-thaw motility and plasma membrane integrity of 12.7 ± 1.9% and 22.7 ± 5.4%, respectively, while kangaroo spermatozoa frozen by ultra-rapid freezing techniques showed no evidence of post-thaw viability. The use of 10–20% DMA represents a modest but significant improvement in the development of a sperm cryopreservation procedure for kangaroos.
© 2008 Published by Elsevier Inc.
Keyword Kangaroo
Cauda epididymidal spermatozoa
Glycerol
Dimethylsulphoxide (DMSO)
Dimethylacetamide (DMA)
Immediate dilution post-thaw
Vitrification
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
 
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Created: Tue, 31 Mar 2009, 21:22:05 EST by Mrs Kathy Bachmann on behalf of School of Animal Studies