In vitro and in vivo imaging of xenobiotic transport in human skin and in the rat liver

Roberts, Michael S., Roberts, Matthew J., Robertson, Thomas A., Sanchez, Washington, Thorling, Camilla, Zou, Yuhong, Zhao, Xin, Becker, Wolfgang and Zvyagin, Andrei V. (2008) In vitro and in vivo imaging of xenobiotic transport in human skin and in the rat liver. Journal of Biophotonics, 1 6: 478-493. doi:10.1002/jbio.200810058


Author Roberts, Michael S.
Roberts, Matthew J.
Robertson, Thomas A.
Sanchez, Washington
Thorling, Camilla
Zou, Yuhong
Zhao, Xin
Becker, Wolfgang
Zvyagin, Andrei V.
Title In vitro and in vivo imaging of xenobiotic transport in human skin and in the rat liver
Formatted title
In vitro and in vivo imaging of xenobiotic transport in human skin and in the rat liver
Journal name Journal of Biophotonics   Check publisher's open access policy
ISSN 1864-063X
1864-0648
Publication date 2008-12-01
Year available 2008
Sub-type Article (original research)
DOI 10.1002/jbio.200810058
Open Access Status
Volume 1
Issue 6
Start page 478
End page 493
Total pages 16
Editor J. Popp
Place of publication Berlin, Germany
Publisher Wiley - VCH Verlag
Language eng
Subject C1
970106 Expanding Knowledge in the Biological Sciences
020501 Classical and Physical Optics
020504 Photonics, Optoelectronics and Optical Communications
110307 Gastroenterology and Hepatology
Abstract Multiphoton tomography was used to examine xenobiotic transport in vivo. We used the photochemical properties of zinc oxide and fluorescein and multiphoton tomography to study their transport in the skin and in the rat liver in vivo. Zinc oxide nanoparticles were visualised in human skin using the photoluminescence properties of zinc oxide and either a selective emission wavelength band pass filter or a filter with fluorescence lifetime imaging (FLIM). Zinc oxide nanoparticles (30 nm) did not penetrate into human skin in vitro and in vivo and this was validated by scanning electron microscopy with Xray photoelectron spectroscopy. Fluorescein was measured in the liver using FLIM. Fluorescein is rapidly extracted from the blood into the liver cells and then transported into the bile. It is suggested that multipho-
Formatted abstract
Multiphoton tomography was used to examine xenobiotic transport in vivo. We used the photochemical properties of zinc oxide and fluorescein and multiphoton tomography to study their transport in the skin and in the rat liver in vivo. Zinc oxide nanoparticles were visualised in human skin using the photoluminescence properties of zinc oxide and either a selective emission wavelength band pass filter or a filter with fluorescence lifetime imaging (FLIM). Zinc oxide nanoparticles (30 nm) did not penetrate into human skin in vitro and in vivo and this was validated by scanning electron microscopy with X-ray photoelectron spectroscopy. Fluorescein was measured in the liver using FLIM. Fluorescein is rapidly extracted from the blood into the liver cells and then transported into the bile. It is suggested that multiphoton tomography may be of particular use in defining in vivo 4D (in both space and time) pharmacokinetics.
© 2008 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Keyword Multi-photon tomography
Fluorescence lifetime imaging
Pharmacokinetics
Zinc oxide nanoparticle
Fluorescein
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Abstracted from a lecture presented at FLIM2008, Saarbrucken, Germany, June 2008

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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Created: Tue, 31 Mar 2009, 02:41:29 EST by Maree Knight on behalf of School of Medicine