Recombinant EPF/chaperonin 10 promotes the survival of O4-positive pro-oligodendrocytes prepared from neonatal rat brain

McCombe, P. A. (2008) Recombinant EPF/chaperonin 10 promotes the survival of O4-positive pro-oligodendrocytes prepared from neonatal rat brain. Cell Stress & Chaperones, 13 4: 467-474. doi:10.1007/s12192-008-0045-1


Author McCombe, P. A.
Title Recombinant EPF/chaperonin 10 promotes the survival of O4-positive pro-oligodendrocytes prepared from neonatal rat brain
Journal name Cell Stress & Chaperones   Check publisher's open access policy
ISSN 1355-8145
1466-1268
Publication date 2008-12-01
Year available 2008
Sub-type Article (original research)
DOI 10.1007/s12192-008-0045-1
Open Access Status DOI
Volume 13
Issue 4
Start page 467
End page 474
Total pages 8
Editor L.E. Hightower
Place of publication Dordrecht, Netherlands
Publisher Springer Netherlands
Language eng
Subject C1
110902 Cellular Nervous System
C Society
920111 Nervous System and Disorders
Abstract Chaperonin 10 (cpn 10) is a small heat-shock protein that is usually intracellular. Early pregnancy factor (EPF), a biologically active protein that was first described in the serum of pregnant mammals, is homologous to cpn 10. EPF/cpn 10 has been reported to have effects on immunomodulation and cell survival and to inhibit activation of toll-like receptors by lipopolysaccharide. We found that recombinant EPF/cpn 10 was able to suppress experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis, which is a disease causing inflammation and demyelination of the brain and spinal cord. This beneficial effect could be due to anti-inflammatory and/or cell survival properties of EPF/cpn 10. We aimed to assess the effects of cpn 10 on cells of the oligodendrocyte lineage because oligodendrocytes are the brain cells that produce myelin and that are depleted in multiple sclerosis. Two forms of recombinant EPF/cpn 10 were prepared in the pGEX expression system and in the baculovirus expression system. Purified O4+ pro-oligodendrocytes were prepared from the brains of day-old Wistar rats and isolated by cell sorting with flow cytometry. Single cells were dispensed into micro-well plates and tested for survival in the presence of a range of concentrations of the two forms of cpn 10. We also studied the effects of bFGF, PDGF, IGF-1 and insulin as controls. With cpn 10 present, there was enhanced survival of O4+ cells.
Keyword Oligodendrocyte
Multiple sclerosis
cpn 10
Growth factors
Early pregnancy factor
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
UQ Centre for Clinical Research Publications
 
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Created: Tue, 31 Mar 2009, 02:14:08 EST by Carmen Buttery on behalf of UQ Centre for Clinical Research