Differential subnuclear localisation of hnRNPs A/B is dependent on transcription and cell cycle stage

Friend, Lexie R., Han, Siew Ping, Rothnagel, Joseph A. and Smith, Ross (2008) Differential subnuclear localisation of hnRNPs A/B is dependent on transcription and cell cycle stage. Biochimica et Biophysica Acta - Molecular Cell Research, 1783 10: 1972-1980. doi:10.1016/j.bbamcr.2008.05.021


Author Friend, Lexie R.
Han, Siew Ping
Rothnagel, Joseph A.
Smith, Ross
Title Differential subnuclear localisation of hnRNPs A/B is dependent on transcription and cell cycle stage
Journal name Biochimica et Biophysica Acta - Molecular Cell Research   Check publisher's open access policy
ISSN 0167-4889
Publication date 2008-06-06
Year available 2008
Sub-type Article (original research)
DOI 10.1016/j.bbamcr.2008.05.021
Open Access Status
Volume 1783
Issue 10
Start page 1972
End page 1980
Total pages 9
Place of publication Dordrecht, Netherlands
Publisher Elsevier
Language eng
Subject C1
060199 Biochemistry and Cell Biology not elsewhere classified
970106 Expanding Knowledge in the Biological Sciences
Abstract The heterogeneous nuclear ribonucleoproteins A1, A2/B1 and A3 (hnRNPs A/B) are involved in many nuclear functions that are confined to distinct regions within the nucleus. To characterise and compare the distribution of the hnRNPs A/B in these subnuclear compartments, their colocalisation with spliceosomal components, nascent transcripts and other nuclear markers in HeLa cells was investigated by immunostaining and transfection of GFP constructs. The mechanisms of this localisation were further explored by treating cells with detergent, nucleases and transcription inhibitors. We have also examined the dynamics of A2/B1 throughout the cell cycle. Our results show that hnRNPs A/B have different subnuclear localisations, with A1 differentially localised to the nuclear envelope, and A2/B1 and A3 enriched around nucleoli. This pattern of distribution was dependent on RNA integrity and active transcription. The hnRNPs A/B preferentially colocalised with a subset of splicing factors. Significantly, only rarely did transcription factories colocalise with high levels of these hnRNPs. Moreover, localisation of A2/B1 changed with cell cycle stage. Our findings show that the subnuclear localisation of the hnRNPs A/B is differentially, spatially and temporally regulated, and suggest that this localisation may be relevant to their nuclear functions.
Keyword hnRNP A/B
Spliceosome
Splicing Factor
Transcription inhibition
Immunoprecipitation
Cell cycle
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 6 June 2008

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
 
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Created: Tue, 31 Mar 2009, 01:35:11 EST by Glenda Chown on behalf of School of Chemistry & Molecular Biosciences