Identification of claudin-4 as a marker highly overexpressed in both primary and metastatic prostate cancer

Kelly Anne Landers, Samaratunga, Hemamali, Linda Teng, Buck, M., Burger, Michelle J., Betty Scells, Lavin, Martin F. and Gardiner, Robert A. (2008) Identification of claudin-4 as a marker highly overexpressed in both primary and metastatic prostate cancer. British Journal of Cancer, 99 3: 491-501. doi:10.1038/sj.bjc.6604486


Author Kelly Anne Landers
Samaratunga, Hemamali
Linda Teng
Buck, M.
Burger, Michelle J.
Betty Scells
Lavin, Martin F.
Gardiner, Robert A.
Title Identification of claudin-4 as a marker highly overexpressed in both primary and metastatic prostate cancer
Journal name British Journal of Cancer   Check publisher's open access policy
ISSN 0007-0920
1532-1827
Publication date 2008-01-01
Year available 2008
Sub-type Article (original research)
DOI 10.1038/sj.bjc.6604486
Open Access Status DOI
Volume 99
Issue 3
Start page 491
End page 501
Total pages 11
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Subject 2730 Oncology
1306 Cancer Research
Abstract In the quest for markers of expression and progression for prostate cancer (PCa), the majority of studies have focussed on molecular data exclusively from primary tumours. Although expression in metastases is inferred, a lack of correlation with secondary tumours potentially limits their applicability diagnostically and therapeutically. Molecular targets were identified by examining expression profiles of prostate cell lines using cDNA microarrays. Those genes identified were verified on PCa cell lines and tumour samples from both primary and secondary tumours using real-time RT-PCR, western blotting and immunohistochemistry. Claudin-4, coding for an integral membrane cell-junction protein, was the most significantly (P<0.00001) upregulated marker in both primary and metastatic tumour specimens compared with benign prostatic hyperplasia at both RNA and protein levels. In primary tumours, claudin-4 was more highly expressed in lower grade (Gleason 6) lesions than in higher grade ( Gleason >= 7) cancers. Expression was prominent throughout metastases from a variety of secondary sites in fresh-frozen and formalin-fixed specimens from both androgen-intact and androgen-suppressed patients. As a result of its prominent expression in both primary and secondary PCas, together with its established role as a receptor for Clostridium perfringens enterotoxin, claudin-4 may be useful as a potential marker and therapeutic target for PCa metastases.
Keyword Oncology
Oncology
ONCOLOGY
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
UQ Centre for Clinical Research Publications
 
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Created: Tue, 31 Mar 2009, 00:37:23 EST by Carmen Buttery on behalf of UQ Centre for Clinical Research