Imaging of zinc oxide nanoparticle penetration in human skin in vitro and in vivo

Zvyagin, Andrei V., Zhao, Xin, Gierden, Audrey, Sanchez, Washington, Ross, Justin A. and Roberts, Michael. S. (2008) Imaging of zinc oxide nanoparticle penetration in human skin in vitro and in vivo. Journal of Biomedical Optics, 13 6: 064031-1-064031-9. doi:10.1117/1.3041492

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Author Zvyagin, Andrei V.
Zhao, Xin
Gierden, Audrey
Sanchez, Washington
Ross, Justin A.
Roberts, Michael. S.
Title Imaging of zinc oxide nanoparticle penetration in human skin in vitro and in vivo
Formatted title
Imaging of zinc oxide nanoparticle penetration in human skin in vitro and in vivo
Journal name Journal of Biomedical Optics   Check publisher's open access policy
ISSN 1083-3668
Publication date 2008-01-01
Year available 2008
Sub-type Article (original research)
DOI 10.1117/1.3041492
Open Access Status File (Publisher version)
Volume 13
Issue 6
Start page 064031-1
End page 064031-9
Total pages 9
Editor B. J. Tromberg
Place of publication Bellingham, WA, United States
Publisher S P I E - International Society for Optical Engineering
Language eng
Subject 2504 Electronic, Optical and Magnetic Materials
2502 Biomaterials
3107 Atomic and Molecular Physics, and Optics
2204 Biomedical Engineering
Abstract Zinc oxide (ZnO-nano) and titanium dioxide nanoparticles (20 to 30 nm) are widely used in several topical skin care products, such as sunscreens. However, relatively few studies have addressed the subdermal absorption of these nanoparticles in vivo. We report on investigation of the distribution of topically applied ZnO in excised and in vivo human skin, using multiphoton microscopy (MPM) imaging with a combination of scanning electron microscopy (SEM) and an energy-dispersive x-ray (EDX) technique to determine the level of penetration of nanoparticles into the sub-dermal layers of the skin. The good visualization of ZnO in skin achieved appeared to result from two factors. First, the ZnO principal photoluminescence at 385 nm is in the "quiet" spectral band of skin autofluorescence dominated by the endogenous skin fluorophores, i. e., NAD[P]H and FAD. Second, the two-photon action cross section of ZnO-nano [sigma((TPEF))(ZnO) similar to 0.26 GM; diameter, 18 nm] is high: similar to 500-fold of that inferred from its bulk third-order nonlinear susceptibility [Im chi((3))(ZnO)], and is favorably compared to that of NAD[P]H and FAD. The overall outcome from MPM, SEM, and EDX studies was that, in humans in vivo, ZnO nanoparticles stayed in the stratum corneum (SC) and accumulated into skin folds and/or hair follicle roots of human skin. Given the lack of penetration of these nanoparticles past the SC and that the outermost layers of SC have a good turnover rate, these data suggest that the form of ZnO-nano studied here is unlikely to result in safety concerns. (c) 2008 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3041492]
Keyword nonlinear optical microscopy
nanoparticle toxicity
zinc oxide
transdermal permeability
multiphoton microscopy
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 131 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 31 Mar 2009, 00:25:16 EST by Maree Knight on behalf of School of Medicine